Detailed information of TA system TA231415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 356979..357733
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: LZG50_RS01710
• Protein ID: WP_021560068.1
• Coordinates: 356979..357464 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: LZG50_RS01715
• Protein ID: WP_000801915.1
• Coordinates: 357455..357733 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS01685 (352251)	352251..353081	+	831	WP_000928723.1	rhomboid family intramembrane serine protease GlpG	-
LZG50_RS01690 (353098)	353098..353856	+	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
LZG50_RS01695 (353838)	353838..354452	-	615	Protein_336	DNA-binding transcriptional regulator RtcR	-
LZG50_RS01700 (354671)	354671..355897	+	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
LZG50_RS01705 (355901)	355901..356929	+	1029	WP_024247668.1	RNA 3'-terminal phosphate cyclase	-
LZG50_RS01710 (356979)	356979..357464	-	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
LZG50_RS01715 (357455)	357455..357733	-	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
LZG50_RS01720 (357800)	357800..360505	-	2706	WP_000906934.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T231415 WP_021560068.1 NZ_CP090611:c357464-356979 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T231415 NZ_CP126348:1944823-1944926 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT231415 WP_000801915.1 NZ_CP090611:c357733-357455 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT231415 NZ_CP126348:c1944964-1944693 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCTAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A822TTG1