Detailed information of TA system TA231414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 319997..320592
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A826P6U8
• Locus tag: LZG50_RS01545
• Protein ID: WP_000155160.1
• Coordinates: 320215..320592 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A827G8B6
• Locus tag: LZG50_RS01540
• Protein ID: WP_000557316.1
• Coordinates: 319997..320218 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS01515 (315073)	315073..316182	+	1110	WP_001297972.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LZG50_RS01520 (316230)	316230..317156	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LZG50_RS01525 (317153)	317153..318430	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
LZG50_RS01530 (318427)	318427..319194	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LZG50_RS01535 (319196)	319196..319909	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LZG50_RS01540 (319997)	319997..320218	+	222	WP_000557316.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LZG50_RS01545 (320215)	320215..320592	+	378	WP_000155160.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LZG50_RS01550 (320801)	320801..322117	+	1317	WP_000803580.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LZG50_RS01555 (322215)	322215..323102	+	888	WP_000099282.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LZG50_RS01560 (323099)	323099..323944	+	846	WP_000572184.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LZG50_RS01565 (323946)	323946..325016	+	1071	WP_000907827.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	317153..325756	8603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13945.20 Da
• Isoelectric Point: 6.4830

>T231414 WP_000155160.1 NZ_CP090611:320215-320592 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT

Nucleotide

• Length: 378 bp

>T231414 NZ_CP126348:1792655-1792909 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8106.25 Da
• Isoelectric Point: 5.5759

>AT231414 WP_000557316.1 NZ_CP090611:319997-320218 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK

Nucleotide

• Length: 222 bp

>AT231414 NZ_CP126348:1792407-1792658 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A826P6U8

Antitoxin

• AlphaFold DB: A0A827G8B6