Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 292713..293344 Replicon chromosome
Accession NZ_CP090611
Organism Escherichia coli isolate DCE7

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag LZG50_RS01390 Protein ID WP_001260301.1
Coordinates 292713..292988 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LZG50_RS01395 Protein ID WP_000593555.1
Coordinates 292985..293344 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG50_RS01375 (287717) 287717..288784 + 1068 WP_024242100.1 HlyD family secretion protein -
LZG50_RS01380 (288781) 288781..291516 + 2736 WP_096891200.1 ribosome-associated ATPase/putative transporter RbbA -
LZG50_RS01385 (291516) 291516..292640 + 1125 WP_024242098.1 ABC transporter permease -
LZG50_RS01390 (292713) 292713..292988 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
LZG50_RS01395 (292985) 292985..293344 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LZG50_RS01400 (293464) 293464..293865 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LZG50_RS01405 (293871) 293871..294677 - 807 WP_000173679.1 nickel import ATP-binding protein NikE -
LZG50_RS01410 (294674) 294674..295438 - 765 WP_001136232.1 nickel import ATP-binding protein NikD -
LZG50_RS01415 (295438) 295438..296271 - 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
LZG50_RS01420 (296268) 296268..297212 - 945 WP_000947071.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T231413 WP_001260301.1 NZ_CP090611:292713-292988 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T231413 NZ_CP126348:1792407-1792658 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT231413 WP_000593555.1 NZ_CP090611:292985-293344 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT231413 NZ_CP126348:1792655-1792909 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References