Detailed information of TA system TA231406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1170025..1170645
• Replicon: chromosome
• Accession: NZ_CP090609
• Organism: Burkholderia cepacia strain CMCC(B)23005

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LZ568_RS23110
• Protein ID: WP_167562614.1
• Coordinates: 1170025..1170342 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: LZ568_RS23115
• Protein ID: WP_021157290.1
• Coordinates: 1170346..1170645 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ568_RS23100 (LZ568_23100)	1166561..1167994	-	1434	WP_027790993.1	aldehyde dehydrogenase family protein	-
LZ568_RS23105 (LZ568_23105)	1168024..1169664	-	1641	WP_027790992.1	acetolactate synthase large subunit	-
LZ568_RS23110 (LZ568_23110)	1170025..1170342	+	318	WP_167562614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ568_RS23115 (LZ568_23115)	1170346..1170645	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
LZ568_RS23120 (LZ568_23120)	1170702..1171988	-	1287	WP_059684715.1	DUF445 domain-containing protein	-
LZ568_RS23125 (LZ568_23125)	1172200..1173420	-	1221	WP_059693887.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
LZ568_RS23130 (LZ568_23130)	1173410..1173736	-	327	WP_006479527.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
LZ568_RS23135 (LZ568_23135)	1173757..1174242	-	486	WP_034196026.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
LZ568_RS23140 (LZ568_23140)	1174260..1175531	-	1272	WP_059694307.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-100)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11820.54 Da
• Isoelectric Point: 10.2314

>T231406 WP_167562614.1 NZ_CP090609:1170025-1170342 [Burkholderia cepacia]
MPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
GGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T231406 NZ_CP126347:22992-23273 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT231406 WP_021157290.1 NZ_CP090609:1170346-1170645 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT231406 NZ_CP126347:22751-23002 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3