Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 800784..801361 Replicon chromosome
Accession NZ_CP090609
Organism Burkholderia cepacia strain CMCC(B)23005

Toxin (Protein)


Gene name vapC Uniprot ID A0A4S3H6U0
Locus tag LZ568_RS21480 Protein ID WP_059696512.1
Coordinates 800996..801361 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U2HEK9
Locus tag LZ568_RS21475 Protein ID WP_021160898.1
Coordinates 800784..801002 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ568_RS21450 (LZ568_21450) 796046..796528 + 483 WP_059696503.1 GNAT family N-acetyltransferase -
LZ568_RS21455 (LZ568_21455) 796616..797881 - 1266 WP_059696505.1 Zn-dependent hydrolase -
LZ568_RS21460 (LZ568_21460) 797874..798398 - 525 WP_059696508.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
LZ568_RS21465 (LZ568_21465) 798453..799619 - 1167 WP_027791290.1 branched-chain amino acid ABC transporter substrate-binding protein -
LZ568_RS21470 (LZ568_21470) 799797..800693 + 897 WP_059696510.1 LysR substrate-binding domain-containing protein -
LZ568_RS21475 (LZ568_21475) 800784..801002 + 219 WP_021160898.1 hypothetical protein Antitoxin
LZ568_RS21480 (LZ568_21480) 800996..801361 + 366 WP_059696512.1 type II toxin-antitoxin system VapC family toxin Toxin
LZ568_RS21485 (LZ568_21485) 801701..802264 + 564 WP_006488685.1 alkyl hydroperoxide reductase subunit C -
LZ568_RS21490 (LZ568_21490) 802393..803991 + 1599 WP_059696513.1 alkyl hydroperoxide reductase subunit F -
LZ568_RS21495 (LZ568_21495) 804086..804877 - 792 WP_059696515.1 hypothetical protein -
LZ568_RS21500 (LZ568_21500) 805038..805592 - 555 WP_059696558.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13420.45 Da        Isoelectric Point: 8.4389

>T231405 WP_059696512.1 NZ_CP090609:800996-801361 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLAGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Download         Length: 366 bp

>T231405 NZ_CP126346:c84042-83755 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGATTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.51 Da        Isoelectric Point: 5.0604

>AT231405 WP_021160898.1 NZ_CP090609:800784-801002 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Download         Length: 219 bp

>AT231405 NZ_CP126346:c84290-84039 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGTCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S3H6U0

Antitoxin

Source ID Structure
AlphaFold DB U2HEK9

References