Detailed information of TA system TA231395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3100135..3100769
• Replicon: chromosome
• Accession: NZ_CP090597
• Organism: Pectobacterium aroidearum strain QJ002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y24_RS13775
• Protein ID: WP_256598648.1
• Coordinates: 3100135..3100539 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: L0Y24_RS13780
• Protein ID: WP_012765080.1
• Coordinates: 3100539..3100769 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y24_RS13755 (L0Y24_13735)	3096698..3096901	-	204	WP_015840819.1	protein DsrB	-
L0Y24_RS13760 (L0Y24_13740)	3097112..3098527	+	1416	WP_181844696.1	diguanylate cyclase	-
L0Y24_RS13765 (L0Y24_13745)	3098803..3099021	+	219	WP_015840821.1	KTSC domain-containing protein	-
L0Y24_RS13770 (L0Y24_13750)	3099241..3099597	+	357	WP_256598647.1	hypothetical protein	-
L0Y24_RS13775 (L0Y24_13755)	3100135..3100539	-	405	WP_256598648.1	tRNA(fMet)-specific endonuclease VapC	Toxin
L0Y24_RS13780 (L0Y24_13760)	3100539..3100769	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y24_RS13785 (L0Y24_13765)	3100863..3101696	-	834	WP_256598649.1	DUF4942 domain-containing protein	-
L0Y24_RS13790 (L0Y24_13770)	3101814..3102134	-	321	WP_256598650.1	TA system toxin CbtA family protein	-
L0Y24_RS13795 (L0Y24_13775)	3102204..3102539	-	336	WP_256598651.1	type IV toxin-antitoxin system YeeU family antitoxin	-
L0Y24_RS13800 (L0Y24_13780)	3102579..3103052	-	474	WP_256598652.1	DNA repair protein RadC	-
L0Y24_RS13805 (L0Y24_13785)	3103175..3103525	-	351	WP_194430415.1	hypothetical protein	-
L0Y24_RS13810 (L0Y24_13790)	3103583..3104416	-	834	WP_256598653.1	hypothetical protein	-
L0Y24_RS13815 (L0Y24_13795)	3104563..3104721	-	159	WP_189654467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15014.25 Da
• Isoelectric Point: 8.8074

>T231395 WP_256598648.1 NZ_CP090597:c3100539-3100135 [Pectobacterium aroidearum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSINPEKNLAVIEGFSARLEVQNYGFGAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T231395 NZ_CP126345:c2461078-2460902 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT231395 WP_012765080.1 NZ_CP090597:c3100769-3100539 [Pectobacterium aroidearum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT231395 NZ_CP126345:c2460856-2460440 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4