Detailed information of TA system TA231384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3181993..3182627
• Replicon: chromosome
• Accession: NZ_CP090596
• Organism: Pectobacterium aroidearum strain QJ003

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y20_RS14340
• Protein ID: WP_256598648.1
• Coordinates: 3181993..3182397 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: L0Y20_RS14345
• Protein ID: WP_012765080.1
• Coordinates: 3182397..3182627 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y20_RS14320 (L0Y20_14295)	3178556..3178759	-	204	WP_015840819.1	protein DsrB	-
L0Y20_RS14325 (L0Y20_14300)	3178970..3180385	+	1416	WP_181844696.1	diguanylate cyclase	-
L0Y20_RS14330 (L0Y20_14305)	3180661..3180879	+	219	WP_015840821.1	KTSC domain-containing protein	-
L0Y20_RS14335 (L0Y20_14310)	3181099..3181455	+	357	WP_256598647.1	hypothetical protein	-
L0Y20_RS14340 (L0Y20_14315)	3181993..3182397	-	405	WP_256598648.1	tRNA(fMet)-specific endonuclease VapC	Toxin
L0Y20_RS14345 (L0Y20_14320)	3182397..3182627	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y20_RS14350 (L0Y20_14325)	3182721..3183554	-	834	WP_256598649.1	DUF4942 domain-containing protein	-
L0Y20_RS14355 (L0Y20_14330)	3183672..3183992	-	321	WP_256598650.1	TA system toxin CbtA family protein	-
L0Y20_RS14360 (L0Y20_14335)	3184062..3184397	-	336	WP_256598651.1	type IV toxin-antitoxin system YeeU family antitoxin	-
L0Y20_RS14365 (L0Y20_14340)	3184437..3184910	-	474	WP_256598652.1	DNA repair protein RadC	-
L0Y20_RS14370 (L0Y20_14345)	3185033..3185383	-	351	WP_194430415.1	hypothetical protein	-
L0Y20_RS14375 (L0Y20_14350)	3185441..3186274	-	834	WP_256598653.1	hypothetical protein	-
L0Y20_RS14380 (L0Y20_14355)	3186421..3186579	-	159	WP_189654467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15014.25 Da
• Isoelectric Point: 8.8074

>T231384 WP_256598648.1 NZ_CP090596:c3182397-3181993 [Pectobacterium aroidearum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSINPEKNLAVIEGFSARLEVQNYGFGAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T231384 NZ_CP126345:289410-289937 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT231384 WP_012765080.1 NZ_CP090596:c3182627-3182397 [Pectobacterium aroidearum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT231384 NZ_CP126345:289137-289403 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4