Detailed information of TA system TA231373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3169092..3169726
• Replicon: chromosome
• Accession: NZ_CP090595
• Organism: Pectobacterium aroidearum strain QJ011

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y21_RS14225
• Protein ID: WP_256598648.1
• Coordinates: 3169092..3169496 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: L0Y21_RS14230
• Protein ID: WP_012765080.1
• Coordinates: 3169496..3169726 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y21_RS14205 (L0Y21_14185)	3165655..3165858	-	204	WP_015840819.1	protein DsrB	-
L0Y21_RS14210 (L0Y21_14190)	3166069..3167484	+	1416	WP_181844696.1	diguanylate cyclase	-
L0Y21_RS14215 (L0Y21_14195)	3167760..3167978	+	219	WP_015840821.1	KTSC domain-containing protein	-
L0Y21_RS14220 (L0Y21_14200)	3168198..3168554	+	357	WP_256598647.1	hypothetical protein	-
L0Y21_RS14225 (L0Y21_14205)	3169092..3169496	-	405	WP_256598648.1	tRNA(fMet)-specific endonuclease VapC	Toxin
L0Y21_RS14230 (L0Y21_14210)	3169496..3169726	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y21_RS14235 (L0Y21_14215)	3169820..3170653	-	834	WP_256598649.1	DUF4942 domain-containing protein	-
L0Y21_RS14240 (L0Y21_14220)	3170771..3171091	-	321	WP_256598650.1	TA system toxin CbtA family protein	-
L0Y21_RS14245 (L0Y21_14225)	3171161..3171496	-	336	WP_256598651.1	type IV toxin-antitoxin system YeeU family antitoxin	-
L0Y21_RS14250 (L0Y21_14230)	3171536..3172009	-	474	WP_256598652.1	DNA repair protein RadC	-
L0Y21_RS14255 (L0Y21_14235)	3172132..3172482	-	351	WP_194430415.1	hypothetical protein	-
L0Y21_RS14260 (L0Y21_14240)	3172540..3173373	-	834	WP_256598653.1	hypothetical protein	-
L0Y21_RS14265 (L0Y21_14245)	3173520..3173678	-	159	WP_189654467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15014.25 Da
• Isoelectric Point: 8.8074

>T231373 WP_256598648.1 NZ_CP090595:c3169496-3169092 [Pectobacterium aroidearum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSINPEKNLAVIEGFSARLEVQNYGFGAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T231373 NZ_CP126343:c2442739-2442563 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT231373 WP_012765080.1 NZ_CP090595:c3169726-3169496 [Pectobacterium aroidearum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT231373 NZ_CP126343:c2442517-2442101 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4