Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1397722..1398517 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ECH74115_RS07390 Protein ID WP_000854914.1
Coordinates 1398143..1398517 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag ECH74115_RS07385 Protein ID WP_001280954.1
Coordinates 1397722..1398096 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS07345 1393077..1393982 + 906 WP_000203541.1 chemotaxis protein -
ECH74115_RS07350 1393979..1395049 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
ECH74115_RS07360 1395389..1396207 + 819 WP_001234682.1 DUF945 domain-containing protein -
ECH74115_RS07365 1396298..1396783 + 486 WP_000214398.1 antirestriction protein -
ECH74115_RS07370 1396799..1397275 + 477 WP_001186738.1 RadC family protein -
ECH74115_RS07375 1397338..1397559 + 222 WP_001220314.1 DUF987 domain-containing protein -
ECH74115_RS07385 1397722..1398096 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECH74115_RS07390 1398143..1398517 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ECH74115_RS07395 1398514..1399005 + 492 WP_000976853.1 hypothetical protein -
ECH74115_RS07400 1399017..1399214 + 198 WP_000839282.1 DUF957 domain-containing protein -
ECH74115_RS07405 1399299..1400141 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ECH74115_RS07415 1400612..1401550 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
ECH74115_RS07420 1401605..1402342 + 738 WP_000283664.1 zinc-binding phosphatase -
ECH74115_RS07425 1402366..1402920 + 555 WP_001001917.1 molecular chaperone YcdY -
ECH74115_RS07430 1403022..1403513 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T23136 WP_000854914.1 NC_011353:1398143-1398517 [Escherichia coli O157:H7 str. EC4115]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T23136 NC_011353:1398143-1398517 [Escherichia coli O157:H7 str. EC4115]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT23136 WP_001280954.1 NC_011353:1397722-1398096 [Escherichia coli O157:H7 str. EC4115]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT23136 NC_011353:1397722-1398096 [Escherichia coli O157:H7 str. EC4115]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References