Detailed information of TA system TA231359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3768926..3769452
• Replicon: chromosome
• Accession: NZ_CP090594
• Organism: Pectobacterium aroidearum strain QJ034

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: L0Y22_RS16825
• Protein ID: WP_256542272.1
• Coordinates: 3769147..3769452 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: L0Y22_RS16820
• Protein ID: WP_198482245.1
• Coordinates: 3768926..3769144 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y22_RS16790	3764300..3764431	-	132	WP_256542269.1	hypothetical protein	-
L0Y22_RS16800 (L0Y22_16775)	3765178..3766437	+	1260	WP_256542270.1	integrase arm-type DNA-binding domain-containing protein	-
L0Y22_RS16805 (L0Y22_16780)	3766582..3766752	-	171	WP_164669135.1	hypothetical protein	-
L0Y22_RS16810 (L0Y22_16785)	3766893..3767234	+	342	WP_256542271.1	hypothetical protein	-
L0Y22_RS16815 (L0Y22_16790)	3768124..3768867	+	744	WP_198482246.1	MobC family replication-relaxation protein	-
L0Y22_RS16820 (L0Y22_16795)	3768926..3769144	+	219	WP_198482245.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
L0Y22_RS16825 (L0Y22_16800)	3769147..3769452	+	306	WP_256542272.1	type II toxin-antitoxin system toxin CcdB	Toxin
L0Y22_RS16830 (L0Y22_16805)	3769837..3770880	-	1044	WP_256542273.1	DUF4435 domain-containing protein	-
L0Y22_RS16835 (L0Y22_16810)	3771081..3771629	-	549	WP_256620710.1	hypothetical protein	-
L0Y22_RS16840 (L0Y22_16815)	3771725..3772516	-	792	WP_256620711.1	hypothetical protein	-
L0Y22_RS16845 (L0Y22_16820)	3772988..3773200	-	213	WP_256542275.1	hypothetical protein	-
L0Y22_RS16850 (L0Y22_16825)	3773526..3773768	-	243	WP_205947008.1	helix-turn-helix transcriptional regulator	-
L0Y22_RS16855 (L0Y22_16830)	3773872..3774126	+	255	WP_039285388.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3765001..3773437	8436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11690.53 Da
• Isoelectric Point: 4.6761

>T231359 WP_256542272.1 NZ_CP090594:3769147-3769452 [Pectobacterium aroidearum]
MQFIVYEYKRASHYKMFVDVQSDIVDTPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEAI
ADLGDYADEVKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T231359 NZ_CP126341:c4952574-4952203 [Enterobacter cloacae]
ATGAATAATATGGCCGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTCGAAGCTGCGGATGCGATTGA
TCATGCCGCGTCACATCGAGCCATTAGCTTAATTACCTGGATGGAGGTGATGGTGGGCGCACGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTCGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTGCGT
GAAAAGCATGGAATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGCAGAAAATGTCCGTTGATTTCTCGAAA
TACGAAAGATTTTTCGGGTATTGATGAGGTCGTTACGCCTTATCAACTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8174.14 Da
• Isoelectric Point: 7.2038

>AT231359 WP_198482245.1 NZ_CP090594:3768926-3769144 [Pectobacterium aroidearum]
MKHRVSVTVDKDSYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT231359 NZ_CP126341:c4952819-4952577 [Enterobacter cloacae]
ATGAGTGTTTTGGCGGAACTGGATATGGGCAGAATACTGATCGATTTATCTGATGACGTGATTCAACGGCTGGATAACCT
CAAGCAACTGCGAAATCAGCCGCGCGCTGAGCTGCTACGGGAGGCGATAGAGCTGTATCTCGACCAGCAGAGTTCGTCTG
TTATCCGCGATGCGCTTGGCTTGTGGGGAGACAAGAAAGAAGACGGGCTGGAGTATGAACGTAAGCTGCGTGAGGAATGG
TGA