Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3533897..3534522 Replicon chromosome
Accession NZ_CP090594
Organism Pectobacterium aroidearum strain QJ034

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag L0Y22_RS15820 Protein ID WP_005976087.1
Coordinates 3534319..3534522 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C6DB73
Locus tag L0Y22_RS15815 Protein ID WP_005976089.1
Coordinates 3533897..3534265 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Y22_RS15800 (L0Y22_15780) 3529528..3532656 + 3129 WP_015839362.1 efflux RND transporter permease subunit -
L0Y22_RS15805 (L0Y22_15785) 3532891..3533139 + 249 WP_015839361.1 type B 50S ribosomal protein L31 -
L0Y22_RS15810 (L0Y22_15790) 3533157..3533300 + 144 WP_005976091.1 type B 50S ribosomal protein L36 -
L0Y22_RS15815 (L0Y22_15795) 3533897..3534265 + 369 WP_005976089.1 Hha toxicity modulator TomB Antitoxin
L0Y22_RS15820 (L0Y22_15800) 3534319..3534522 + 204 WP_005976087.1 HHA domain-containing protein Toxin
L0Y22_RS15830 (L0Y22_15810) 3535269..3535589 + 321 WP_015839360.1 MGMT family protein -
L0Y22_RS15835 (L0Y22_15815) 3535609..3536190 - 582 WP_256542207.1 YbaY family lipoprotein -
L0Y22_RS15840 (L0Y22_15820) 3536403..3537266 + 864 WP_015839358.1 acyl-CoA thioesterase II -
L0Y22_RS15845 (L0Y22_15825) 3537396..3538706 + 1311 WP_225182003.1 IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3537396..3538706 1310


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T231358 WP_005976087.1 NZ_CP090594:3534319-3534522 [Pectobacterium aroidearum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T231358 NZ_CP126341:c4195658-4195167 [Enterobacter cloacae]
ATGATCTCTGCCCCAGAGCCCCTTCACGCCGGTCATATTATTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
TGGCTTATTACTCGCTGGCATCCAGTGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTTGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAAGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14032.77 Da        Isoelectric Point: 4.4787

>AT231358 WP_005976089.1 NZ_CP090594:3533897-3534265 [Pectobacterium aroidearum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Download         Length: 369 bp

>AT231358 NZ_CP126341:c4195948-4195655 [Enterobacter cloacae]
ATGCCAGCTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAGGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7Z3G5

References