Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1234668..1235293 Replicon chromosome
Accession NZ_CP090592
Organism Pectobacterium aroidearum strain QJ311

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag L0Y27_RS05340 Protein ID WP_005976087.1
Coordinates 1234668..1234871 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C6DB73
Locus tag L0Y27_RS05345 Protein ID WP_005976089.1
Coordinates 1234925..1235293 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Y27_RS05310 (L0Y27_05300) 1230109..1230447 + 339 WP_002208627.1 P-II family nitrogen regulator -
L0Y27_RS05315 (L0Y27_05305) 1230485..1231771 + 1287 WP_015839357.1 ammonium transporter AmtB -
L0Y27_RS05320 (L0Y27_05310) 1231925..1232788 - 864 WP_015839358.1 acyl-CoA thioesterase II -
L0Y27_RS05325 (L0Y27_05315) 1233001..1233582 + 582 WP_181830022.1 YbaY family lipoprotein -
L0Y27_RS05330 (L0Y27_05320) 1233602..1233922 - 321 WP_015839360.1 MGMT family protein -
L0Y27_RS05340 (L0Y27_05330) 1234668..1234871 - 204 WP_005976087.1 HHA domain-containing protein Toxin
L0Y27_RS05345 (L0Y27_05335) 1234925..1235293 - 369 WP_005976089.1 Hha toxicity modulator TomB Antitoxin
L0Y27_RS05350 (L0Y27_05340) 1235890..1236033 - 144 WP_005976091.1 type B 50S ribosomal protein L36 -
L0Y27_RS05355 (L0Y27_05345) 1236051..1236299 - 249 WP_181830023.1 type B 50S ribosomal protein L31 -
L0Y27_RS05360 (L0Y27_05350) 1236534..1239662 - 3129 WP_256599595.1 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T231336 WP_005976087.1 NZ_CP090592:c1234871-1234668 [Pectobacterium aroidearum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T231336 NZ_CP126337:25204-25479 [Proteus vulgaris]
ATGGATCATTATCGCATTGAATACACCAAACGATTTCAAAAAGAAATAAAAAAAATTGATAAATATCACCAAAAAATTAT
TCAAAACTATATTGAAAAACATATTGATAACAGCATTAACCCTCGATTAATTGGAAAAATATTAAAAGGAGATAAAGCGG
AATTTATACGATATCGGATTGGTGATTATCGATTAATCGCAACCATTGAAGATGATAAATTACTGATTTTGGCGCTTTCC
ATTGGGCATCGAAAAGATATTTATAAACATTTTTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14032.77 Da        Isoelectric Point: 4.4787

>AT231336 WP_005976089.1 NZ_CP090592:c1235293-1234925 [Pectobacterium aroidearum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Download         Length: 369 bp

>AT231336 NZ_CP126337:24971-25195 [Proteus vulgaris]
ATGCGAAAAAATTCTACTAAAGCGATTCGATTAAATGATGATGAATTAACTCTTTTTGAATCATATGCCAACGCCAAAGG
ATTAACCTTTAGCGAATTATGTAAAAGTGCTGTTATTGAAAAAATCGAAGATGAAATTGATTTAAAACTTGCTGAGGAAT
CTTATAACGAATATTTAAACGATAATGAAACTTTAAGCCATAAAGCGCTTTTTGATTCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7Z3G5

References