Detailed information of TA system TA231335

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 759929..760589
• Replicon: chromosome
• Accession: NZ_CP090592
• Organism: Pectobacterium aroidearum strain QJ311

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: L0Y27_RS03310
• Protein ID: WP_043881709.1
• Coordinates: 760215..760589 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C6D8Y5
• Locus tag: L0Y27_RS03305
• Protein ID: WP_012773340.1
• Coordinates: 759929..760195 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y27_RS03285 (L0Y27_03285)	755921..756925	-	1005	WP_181845151.1	LacI family DNA-binding transcriptional regulator	-
L0Y27_RS03290 (L0Y27_03290)	756969..757577	-	609	WP_181829659.1	HD domain-containing protein	-
L0Y27_RS03295 (L0Y27_03295)	757990..758637	+	648	WP_181829658.1	hemolysin III family protein	-
L0Y27_RS03300 (L0Y27_03300)	758692..759693	-	1002	WP_194431591.1	tRNA-modifying protein YgfZ	-
L0Y27_RS03305 (L0Y27_03305)	759929..760195	+	267	WP_012773340.1	FAD assembly factor SdhE	Antitoxin
L0Y27_RS03310 (L0Y27_03310)	760215..760589	+	375	WP_043881709.1	protein YgfX	Toxin
L0Y27_RS03315 (L0Y27_03315)	760659..761594	+	936	WP_225181483.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
L0Y27_RS03320 (L0Y27_03320)	761640..761963	-	324	WP_180777498.1	hypothetical protein	-
L0Y27_RS03325 (L0Y27_03325)	761992..762510	-	519	WP_012773344.1	flavodoxin FldB	-
L0Y27_RS03330 (L0Y27_03330)	762673..764001	-	1329	WP_181845156.1	MFS transporter	-
L0Y27_RS03335 (L0Y27_03335)	764313..765212	+	900	WP_256599406.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14673.34 Da
• Isoelectric Point: 10.6585

>T231335 WP_043881709.1 NZ_CP090592:760215-760589 [Pectobacterium aroidearum]
MQLLSLIVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDRQQLWLASDSMGDDEWRHLRQLLLQQKNWAR

Nucleotide

• Length: 375 bp

>T231335 NZ_CP126336:c104527-104195 [Proteus vulgaris]
ATGGTATCTCGCTACGTGCCTGATTCTGGTGATTTGATTTGGATTGATTTTGATCCAGTTGAGGGACATGAGCAGGGCGG
TCATCGTCCTGCTGTTGTCCTCAGTCCATTTGCATACAATAATAAAGTAGGGCTCTTACTATGCGTACCATGTACAACTA
AAGGCAAAGGCTATCCATTTGAATGCGAGATCTCGAACGAAAGAGACAGCTACGCATTAGCTGATCAAGTTACTTGTGTT
GACTGGAGAGCTCGTAAGGTTACTAAAAAAGGCAAAGTGGAAGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.94 Da
• Isoelectric Point: 6.2223

>AT231335 WP_012773340.1 NZ_CP090592:759929-760195 [Pectobacterium aroidearum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLSDSDKRTFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT231335 NZ_CP126336:c104772-104527 [Proteus vulgaris]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCAATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGATAATTTGTTAGCCGGTATCACACCGGAAAATATTCATGAGAAAGTAGATTTCGGAGCTCGCACAGGTAAGGAATTA
ATATAA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E501