Detailed information of TA system TA231324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3777744..3778522
• Replicon: chromosome
• Accession: NZ_CP090591
• Organism: Pectobacterium aroidearum strain QJ313

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: L0Y30_RS16880
• Protein ID: WP_256542279.1
• Coordinates: 3777744..3778232 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: L0Y30_RS16885
• Protein ID: WP_205947014.1
• Coordinates: 3778229..3778522 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y30_RS16845 (L0Y30_16820)	3773004..3773216	-	213	WP_256542275.1	hypothetical protein	-
L0Y30_RS16850 (L0Y30_16825)	3773542..3773784	-	243	WP_205947008.1	helix-turn-helix transcriptional regulator	-
L0Y30_RS16855 (L0Y30_16830)	3773888..3774142	+	255	WP_039285388.1	helix-turn-helix transcriptional regulator	-
L0Y30_RS16860 (L0Y30_16835)	3774228..3774476	+	249	WP_256542276.1	helix-turn-helix transcriptional regulator	-
L0Y30_RS16865 (L0Y30_16840)	3774491..3774709	+	219	WP_256542277.1	type II toxin-antitoxin system antitoxin CcdA	-
L0Y30_RS16870 (L0Y30_16845)	3774757..3775016	+	260	Protein_3297	type II toxin-antitoxin system toxin CcdB	-
L0Y30_RS16875 (L0Y30_16850)	3775397..3777586	-	2190	WP_256542278.1	AAA family ATPase	-
L0Y30_RS16880 (L0Y30_16855)	3777744..3778232	-	489	WP_256542279.1	GNAT family N-acetyltransferase	Toxin
L0Y30_RS16885 (L0Y30_16860)	3778229..3778522	-	294	WP_205947014.1	DUF1778 domain-containing protein	Antitoxin
L0Y30_RS16890 (L0Y30_16865)	3778731..3779687	-	957	WP_256542280.1	AraC family transcriptional regulator	-
L0Y30_RS16895 (L0Y30_16870)	3779803..3780990	+	1188	WP_256542281.1	MFS transporter	-
L0Y30_RS16900 (L0Y30_16875)	3781117..3781539	-	423	WP_012773533.1	MarR family transcriptional regulator	-
L0Y30_RS16905 (L0Y30_16880)	3781700..3782719	+	1020	WP_256542282.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17421.24 Da
• Isoelectric Point: 7.8046

>T231324 WP_256542279.1 NZ_CP090591:c3778232-3777744 [Pectobacterium aroidearum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDVGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMLLMVTLGDLVG
SV

Nucleotide

• Length: 489 bp

>T231324 NZ_CP126331:c49087-48803 [Citrobacter braakii]
GTGTATAAACTCACCGAACGGGCGGCAGAAGACTTCGCCGGGATTTATGATTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACCGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATG
CCGTTCCTGGTGTAATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10892.43 Da
• Isoelectric Point: 10.1968

>AT231324 WP_205947014.1 NZ_CP090591:c3778522-3778229 [Pectobacterium aroidearum]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLTRLDQAPSPN
AALRKTMQTSAPWEQKK

Nucleotide

• Length: 294 bp

>AT231324 NZ_CP126331:c49322-49080 [Citrobacter braakii]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATCAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCAAGTGAAGTTATGCGCTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGCCAGGCTG
TTATTACCGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCAGAAAGAAAACTGAGACATCGTGTA
TAA