Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 549741..550359 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECH74115_RS03200 Protein ID WP_001291435.1
Coordinates 549741..549959 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECH74115_RS03205 Protein ID WP_000344800.1
Coordinates 549985..550359 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS03170 545028..545600 + 573 WP_000779831.1 YbaY family lipoprotein -
ECH74115_RS03175 545633..545944 - 312 WP_000409911.1 MGMT family protein -
ECH74115_RS03180 546323..546676 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECH74115_RS03185 546718..548268 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECH74115_RS03190 548432..548902 - 471 WP_000136192.1 YlaC family protein -
ECH74115_RS03195 549018..549569 - 552 WP_000102553.1 maltose O-acetyltransferase -
ECH74115_RS03200 549741..549959 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECH74115_RS03205 549985..550359 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECH74115_RS03210 550905..554054 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ECH74115_RS03215 554077..555270 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T23131 WP_001291435.1 NC_011353:c549959-549741 [Escherichia coli O157:H7 str. EC4115]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T23131 NC_011353:c549959-549741 [Escherichia coli O157:H7 str. EC4115]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT23131 WP_000344800.1 NC_011353:c550359-549985 [Escherichia coli O157:H7 str. EC4115]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT23131 NC_011353:c550359-549985 [Escherichia coli O157:H7 str. EC4115]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References