Detailed information of TA system TA231280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3165700..3166334
• Replicon: chromosome
• Accession: NZ_CP090588
• Organism: Pectobacterium aroidearum strain AK042

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y25_RS14470
• Protein ID: WP_256553519.1
• Coordinates: 3165700..3166104 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: L0Y25_RS14475
• Protein ID: WP_012765080.1
• Coordinates: 3166104..3166334 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y25_RS14450 (L0Y25_14435)	3162266..3162469	-	204	WP_015840819.1	protein DsrB	-
L0Y25_RS14455 (L0Y25_14440)	3162680..3164095	+	1416	WP_181848683.1	diguanylate cyclase	-
L0Y25_RS14460 (L0Y25_14445)	3164367..3164585	+	219	WP_015840821.1	KTSC domain-containing protein	-
L0Y25_RS14465 (L0Y25_14450)	3164806..3165162	+	357	WP_182100027.1	hypothetical protein	-
L0Y25_RS14470 (L0Y25_14455)	3165700..3166104	-	405	WP_256553519.1	tRNA(fMet)-specific endonuclease VapC	Toxin
L0Y25_RS14475 (L0Y25_14460)	3166104..3166334	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y25_RS14480 (L0Y25_14465)	3166428..3167261	-	834	WP_256553520.1	DUF4942 domain-containing protein	-
L0Y25_RS14485 (L0Y25_14470)	3167379..3167699	-	321	WP_256553521.1	TA system toxin CbtA family protein	-
L0Y25_RS14490 (L0Y25_14475)	3167768..3168103	-	336	WP_249653329.1	type IV toxin-antitoxin system YeeU family antitoxin	-
L0Y25_RS14495 (L0Y25_14480)	3168143..3168616	-	474	WP_249653330.1	DNA repair protein RadC	-
L0Y25_RS14500 (L0Y25_14485)	3168739..3169089	-	351	WP_256553522.1	hypothetical protein	-
L0Y25_RS14505 (L0Y25_14490)	3169147..3169980	-	834	WP_256553523.1	hypothetical protein	-
L0Y25_RS14510 (L0Y25_14495)	3170127..3170285	-	159	WP_155242703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14997.17 Da
• Isoelectric Point: 7.2751

>T231280 WP_256553519.1 NZ_CP090588:c3166104-3165700 [Pectobacterium aroidearum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIDGAEKSVNPEKNLAVIEGFSARLEVQTYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T231280 NZ_CP090588:c3166104-3165700 [Pectobacterium aroidearum]
ATGCTGAAATATCTACTTGATACCAATATCTGTATTTACACCATTAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACACTGATGGAACTAATCGATGGTGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTCGCCGTGATTGAAGGTTTTTCTGCTCGTCTGGAAGTGCAAACGTATGGCTTTGACGCTGCTGTACATACC
GGGCAAATTCGTGCCGAGCTGGCAAGACAAGGAACCCCTATCGGCCCGTATGATGCCATGTTAGCCGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT231280 WP_012765080.1 NZ_CP090588:c3166334-3166104 [Pectobacterium aroidearum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT231280 NZ_CP090588:c3166334-3166104 [Pectobacterium aroidearum]
ATGGAAAAAACCACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCTAAAGCCGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGAACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGACGGGGTAACGCCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4