Detailed information of TA system TA231270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3914673..3915199
• Replicon: chromosome
• Accession: NZ_CP090587
• Organism: Pectobacterium aroidearum strain AK049

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: L0Y26_RS17750
• Protein ID: WP_256553763.1
• Coordinates: 3914894..3915199 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: L0Y26_RS17745
• Protein ID: WP_012773205.1
• Coordinates: 3914673..3914891 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y26_RS17725 (L0Y26_17695)	3910806..3911000	-	195	WP_012773549.1	YlcI/YnfO family protein	-
L0Y26_RS17730 (L0Y26_17700)	3911186..3911527	+	342	WP_256553761.1	hypothetical protein	-
L0Y26_RS17735 (L0Y26_17705)	3911656..3912966	+	1311	WP_180776519.1	IS4 family transposase	-
L0Y26_RS17740 (L0Y26_17710)	3913871..3914614	+	744	WP_256553762.1	MobC family replication-relaxation protein	-
L0Y26_RS17745 (L0Y26_17715)	3914673..3914891	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
L0Y26_RS17750 (L0Y26_17720)	3914894..3915199	+	306	WP_256553763.1	type II toxin-antitoxin system toxin CcdB	Toxin
L0Y26_RS17755 (L0Y26_17725)	3915899..3918355	+	2457	WP_112092901.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3909263..3925456	16193
	-	flank	IS/Tn	-	-	3911656..3912966	1310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11785.63 Da
• Isoelectric Point: 4.8564

>T231270 WP_256553763.1 NZ_CP090587:3914894-3915199 [Pectobacterium aroidearum]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIETHHLSEKVNKTLFPQIHIEGEDYRLMTTELSSVPVEVIGEAI
VDLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T231270 NZ_CP126307:1241630-1242016 [Bosea vestrisii]
ATGATCGTCGATACATCTGCGCTCGTCGCGATCCTTTATCGCGAGCCCGAAGCTGAAGCCTTCGTCAAGCGCATCCACGC
GGCCGAAACCAGCCGGATCAGCGTTGCGAACTATGTCGAGCTATCGATGGTCGTCGAACATCAGCTTGGCCCCGAGGGGA
TGCGCCAAGCCGAAGCGTTCTTCCGGCGTGCAGGGATCATCATCGAGCCGGTGTCCGTGGATCATGGTGATCTGGCGCGT
CAGGCTTTCCTCGATTTCGGCAAGGGGCGCCACCGGGCGGGACTGAATTTCGGCGATTGTTTTGCCTATGCCCTCGCGAA
AGCGAGCGGAGAACCTTTGCTCTTCAAGGGCAATGATTTCTCACAGACCGACATTGCGGCGGCGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT231270 WP_012773205.1 NZ_CP090587:3914673-3914891 [Pectobacterium aroidearum]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT231270 NZ_CP126307:1241385-1241633 [Bosea vestrisii]
ATGAGTCTCAGCATCAAAGATCCCGAAGCCCATCGCCTTGCCCAGGCGATCTCTCATGCCACGGGCGAAAGCATGACCCG
TGTCGTGACCGAAGCCTTGCGGGAACGCTGGAACAAAATCGAACGGCAAAAGGGGCGCGCAAGCGTCGAGGAGCTTCTGC
AGATTGCCGAACGCGCCGCCGGCCATGTGAAGCGCCCATATGCCGACCACGCGGACCTGCTTTATGATGAGAGCGGTATC
CCGAAATGA

Structures

Antitoxin

• AlphaFold DB: E0SK21