Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 32243..32887 Replicon plasmid pEC4115
Accession NC_011351
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag ECH74115_RS29585 Protein ID WP_000833471.1
Coordinates 32705..32887 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag ECH74115_RS29580 Protein ID WP_000466317.1
Coordinates 32243..32680 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS29560 29654..29989 - 336 WP_000963832.1 hypothetical protein -
ECH74115_RS29565 30335..30742 + 408 WP_001190619.1 hypothetical protein -
ECH74115_RS29570 30752..31513 + 762 WP_001097937.1 conjugal transfer protein TraL -
ECH74115_RS29575 31510..32214 + 705 WP_001279867.1 hypothetical protein -
ECH74115_RS29580 32243..32680 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECH74115_RS29585 32705..32887 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
ECH74115_RS29590 33318..33596 - 279 WP_000545934.1 hypothetical protein -
ECH74115_RS29595 33654..33983 - 330 WP_000855122.1 hypothetical protein -
ECH74115_RS29600 34421..34663 - 243 WP_001479502.1 transcriptional regulator -
ECH74115_RS29605 34653..34919 - 267 WP_000868277.1 hypothetical protein -
ECH74115_RS29610 34970..35194 - 225 WP_000749826.1 hypothetical protein -
ECH74115_RS29615 35290..35514 - 225 WP_000866039.1 hypothetical protein -
ECH74115_RS29620 35555..35878 - 324 WP_000432877.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37452 37452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T23127 WP_000833471.1 NC_011351:c32887-32705 [Escherichia coli O157:H7 str. EC4115]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T23127 NC_011351:c32887-32705 [Escherichia coli O157:H7 str. EC4115]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT23127 WP_000466317.1 NC_011351:c32680-32243 [Escherichia coli O157:H7 str. EC4115]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT23127 NC_011351:c32680-32243 [Escherichia coli O157:H7 str. EC4115]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References