Detailed information of TA system TA231266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3165534..3166168
• Replicon: chromosome
• Accession: NZ_CP090587
• Organism: Pectobacterium aroidearum strain AK049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Y26_RS14470
• Protein ID: WP_256553519.1
• Coordinates: 3165534..3165938 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C6C2Z8
• Locus tag: L0Y26_RS14475
• Protein ID: WP_012765080.1
• Coordinates: 3165938..3166168 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y26_RS14450 (L0Y26_14430)	3162100..3162303	-	204	WP_015840819.1	protein DsrB	-
L0Y26_RS14455 (L0Y26_14435)	3162514..3163929	+	1416	WP_181848683.1	diguanylate cyclase	-
L0Y26_RS14460 (L0Y26_14440)	3164201..3164419	+	219	WP_015840821.1	KTSC domain-containing protein	-
L0Y26_RS14465 (L0Y26_14445)	3164640..3164996	+	357	WP_182100027.1	hypothetical protein	-
L0Y26_RS14470 (L0Y26_14450)	3165534..3165938	-	405	WP_256553519.1	tRNA(fMet)-specific endonuclease VapC	Toxin
L0Y26_RS14475 (L0Y26_14455)	3165938..3166168	-	231	WP_012765080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L0Y26_RS14480 (L0Y26_14460)	3166262..3167095	-	834	WP_256553520.1	DUF4942 domain-containing protein	-
L0Y26_RS14485 (L0Y26_14465)	3167213..3167533	-	321	WP_256553521.1	TA system toxin CbtA family protein	-
L0Y26_RS14490 (L0Y26_14470)	3167602..3167937	-	336	WP_249653329.1	type IV toxin-antitoxin system YeeU family antitoxin	-
L0Y26_RS14495 (L0Y26_14475)	3167977..3168450	-	474	WP_249653330.1	DNA repair protein RadC	-
L0Y26_RS14500 (L0Y26_14480)	3168573..3168923	-	351	WP_256553522.1	hypothetical protein	-
L0Y26_RS14505 (L0Y26_14485)	3168981..3169814	-	834	WP_256553523.1	hypothetical protein	-
L0Y26_RS14510 (L0Y26_14490)	3169961..3170119	-	159	WP_155242703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14997.17 Da
• Isoelectric Point: 7.2751

>T231266 WP_256553519.1 NZ_CP090587:c3165938-3165534 [Pectobacterium aroidearum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIDGAEKSVNPEKNLAVIEGFSARLEVQTYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T231266 NZ_CP126275:c94881-94630 [Curtobacterium sp. MCBD17_003]
ATGCGCATCCTCAGCTACTCGGAGACGCGTTCCCACTTCGCCGAGACCCTCAACGCCGTCACGGACGACCGCGAGGAGGT
CGTGGTCACGCGCGCGGGCCACGAATCGGTCGTGCTCGTGTCCCTCGACGACTACGAGTCCCTGAAGGAGACCGCCTACC
TGCTGAGGAACCCCGCGAACGCCCGGCGGCTGCTCGCCTCGATCGAGCGCCTGGAGCGCGGGGAGGGCACGGAGCGGGAC
CTGGACGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8694.75 Da
• Isoelectric Point: 4.3063

>AT231266 WP_012765080.1 NZ_CP090587:c3166168-3165938 [Pectobacterium aroidearum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEDGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT231266 NZ_CP126275:c94633-94373 [Curtobacterium sp. MCBD17_003]
GTGAAGTACGTCTGGGACGACGCTGCCTGGGCCGACTCCGAGTGGTGGCAACAGCAGGACCGGAAGATCGTCAAGCGGAT
CAACGGGCTGCTCCGTGACATCGCGCGCAACGGCAACGAGGGCATCGGCAGACCGGAGCCGCTCAAGCACGGGCTCCGTG
GGTACTGGTCCCGGCGCATCACGGACGAGCACCGTCTCGTGTACCGCGTCACGGACGACAGCGTCCTCATCGCGCAGTGC
CGGTACCACCACGACGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8Q8E4