Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 57878..58437 | Replicon | plasmid pO157 |
| Accession | NC_011350 | ||
| Organism | Escherichia coli O157:H7 str. EC4115 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | ECH74115_RS00330 | Protein ID | WP_000421248.1 |
| Coordinates | 58162..58437 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | ECH74115_RS00325 | Protein ID | WP_001178089.1 |
| Coordinates | 57878..58162 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECH74115_RS00285 | 53069..53629 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| ECH74115_RS00290 | 53762..53974 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| ECH74115_RS00295 | 54219..54680 | + | 462 | WP_001233856.1 | thermonuclease family protein | - |
| ECH74115_RS00300 | 54726..54935 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| ECH74115_RS00305 | 54973..55311 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| ECH74115_RS00310 | 55551..55805 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| ECH74115_RS00315 | 56041..56115 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| ECH74115_RS00320 | 56108..56965 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| ECH74115_RS00325 | 57878..58162 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| ECH74115_RS00330 | 58162..58437 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| ECH74115_RS00335 | 58532..58738 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| ECH74115_RS00340 | 58838..59053 | - | 216 | Protein_64 | transposase | - |
| ECH74115_RS30035 | 59097..59663 | - | 567 | Protein_65 | IS3-like element IS600 family transposase | - |
| ECH74115_RS00350 | 59663..60079 | - | 417 | Protein_66 | IS91 family transposase | - |
| ECH74115_RS00355 | 60079..60264 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| ECH74115_RS00360 | 60441..62651 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| ECH74115_RS00365 | 62695..63084 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG | 1..94644 | 94644 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T23126 WP_000421248.1 NC_011350:58162-58437 [Escherichia coli O157:H7 str. EC4115]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T23126 NC_011350:58162-58437 [Escherichia coli O157:H7 str. EC4115]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT23126 WP_001178089.1 NC_011350:57878-58162 [Escherichia coli O157:H7 str. EC4115]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT23126 NC_011350:57878-58162 [Escherichia coli O157:H7 str. EC4115]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |