Detailed information of TA system TA231255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 6765..7357
• Replicon: plasmid pBY63-4
• Accession: NZ_CP090574
• Organism: Escherichia coli strain BY63

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: LZE53_RS24725
• Protein ID: WP_000520549.1
• Coordinates: 6765..7013 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: LZE53_RS24730
• Protein ID: WP_000681613.1
• Coordinates: 7010..7357 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZE53_RS24695 (LZE53_24605)	3642..4157	+	516	WP_001025395.1	J domain-containing protein	-
LZE53_RS24700 (LZE53_24610)	4262..4804	+	543	WP_001083906.1	hypothetical protein	-
LZE53_RS24705 (LZE53_24615)	4801..5052	+	252	WP_001230707.1	hypothetical protein	-
LZE53_RS24710 (LZE53_24620)	5039..5266	+	228	WP_000801440.1	hypothetical protein	-
LZE53_RS24715 (LZE53_24625)	5720..6019	+	300	WP_000650309.1	hypothetical protein	-
LZE53_RS24720 (LZE53_24630)	6110..6460	+	351	WP_000854259.1	hypothetical protein	-
LZE53_RS24725 (LZE53_24635)	6765..7013	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
LZE53_RS24730 (LZE53_24640)	7010..7357	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LZE53_RS24735 (LZE53_24645)	7718..8221	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
LZE53_RS24740 (LZE53_24650)	8225..9454	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
LZE53_RS24745 (LZE53_24655)	9531..9824	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
LZE53_RS24750 (LZE53_24660)	9821..10036	+	216	WP_000760375.1	EexN family lipoprotein	-
LZE53_RS24755 (LZE53_24665)	10392..11012	+	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
LZE53_RS24760 (LZE53_24670)	11026..11352	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T231255 WP_000520549.1 NZ_CP090574:6765-7013 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T231255 NZ_CP126169:c1474756-1474445 [Rahnella bonaserana]
ATGCATGTTATAGCCAAAGAAGCATTCGACCGGGCGATGCGGCAGTATCCCAATGATAAAACCGCCTTGCTGGCAACCTA
TCGCGGGCTCAAAGAAGGCGACTATCCAACGCCAGATTCCCTGAAATCCGTCTTTTCCAGCCTCGACAATTTCCGCTACC
GGCCAAAATGGTGGGTTATTAACGTCGGCGGCAATAATCTTCGCCTCATTGCTTATATCAATTTCGTCAACAAGCGGGTG
TATGTAAAACATATCGTCACTCATGCGCTTTACGACCAGTTGAACAAACATTACAGGATTTACACACAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT231255 WP_000681613.1 NZ_CP090574:7010-7357 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT231255 NZ_CP126169:c1474448-1474035 [Rahnella bonaserana]
ATGATTGCTGAAGCTATCGAAGCCAGTAAAGCGCTGGTTAAAGCAGTTCCACTGCTGGGAGGAAGTACCAGCACAGCCGA
TTATAAAAGTGCCCTTGAGCTGATTGATTATCTGATTGAGCATGATGACGAAAACCCGCTAATCGAGTTTCTGAGTGCAA
AGATTGAAAGCTATGAGCGCCAGCATACCGATTTCGTCACCTTTAATCAGGCGGTGGGAAATCTGCCACGCGGTGTGGAA
GCACTTCGTCAGCTGATGAAGGATTACGGATTAAAGCAGAACGATCTGCGAGAGGAAATCGGTGGGAAATCACTGGTCAG
TCAGATCCTGAGCGGTAAGCGTTCGCTGACAGTCGAGCATATCCGCGCACTGGCGGTGAGGTTTAATGTACCGACGAGTT
TGTTTATTGACTGA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1