Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 4801..5266 Replicon plasmid pBY63-4
Accession NZ_CP090574
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag LZE53_RS24705 Protein ID WP_001230707.1
Coordinates 4801..5052 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag LZE53_RS24710 Protein ID WP_000801440.1
Coordinates 5039..5266 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS24680 (LZE53_24590) 282..809 - 528 WP_000577043.1 DUF2726 domain-containing protein -
LZE53_RS24685 (LZE53_24595) 944..1348 - 405 WP_012421203.1 hypothetical protein -
LZE53_RS24690 (LZE53_24600) 1361..2227 - 867 WP_001353743.1 replication initiation protein -
LZE53_RS24695 (LZE53_24605) 3642..4157 + 516 WP_001025395.1 J domain-containing protein -
LZE53_RS24700 (LZE53_24610) 4262..4804 + 543 WP_001083906.1 hypothetical protein -
LZE53_RS24705 (LZE53_24615) 4801..5052 + 252 WP_001230707.1 hypothetical protein Toxin
LZE53_RS24710 (LZE53_24620) 5039..5266 + 228 WP_000801440.1 hypothetical protein Antitoxin
LZE53_RS24715 (LZE53_24625) 5720..6019 + 300 WP_000650309.1 hypothetical protein -
LZE53_RS24720 (LZE53_24630) 6110..6460 + 351 WP_000854259.1 hypothetical protein -
LZE53_RS24725 (LZE53_24635) 6765..7013 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
LZE53_RS24730 (LZE53_24640) 7010..7357 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
LZE53_RS24735 (LZE53_24645) 7718..8221 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
LZE53_RS24740 (LZE53_24650) 8225..9454 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
LZE53_RS24745 (LZE53_24655) 9531..9824 + 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
LZE53_RS24750 (LZE53_24660) 9821..10036 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(2-141)

Antitoxin

Flavinator of succinate dehydrogenase

(8-79)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T231254 WP_001230707.1 NZ_CP090574:4801-5052 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T231254 NZ_CP126169:860425-860877 [Rahnella bonaserana]
GTGGCCCTGTGGCGATGTGACCTGAGGGTGTCCTGGCGCAGTCAGCTTTTCTCACTGGGATGTTACGGTGCGGTGATGCT
GCTGATTTTGCTGGCACCCTGGCCTGCGGGTTACTGGCCTGTCTGGATGACGCTGCTGTTGCTGGTGCTGTTTGAGTGCA
TTCGCAGTCAGCGGCGTATCACGGCACGTACTGGTGAAATTGTTCTGACTGATGATTACCGTCTGTTGTGGCGCGGTCAT
GAATGGCAGATTAAACACCCGGTGTGGATGATAAGCCACGGTGCGTTGCTTTCATTGCGTCGGGAGAAGCTCAAAGGCGG
TCTGGCGCAGGCAATGCGCAGCTCAAGACAGCGTTTGTGGCTGGCCTCTGACAGCATGAGTCAGGAAGAGTGGCGGCATT
TGCGCCAGATATTGCTGAATACTCATCCGCGGAGCCCGGCAGAACCGGGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT231254 WP_000801440.1 NZ_CP090574:5039-5266 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT231254 NZ_CP126169:860178-860444 [Rahnella bonaserana]
CTGGATATTAATAACCGTTCCCGAGTTCACTGGGCCTGCCGCCGCGGTATGCGCGAGCTGGATATTTCTATCATGCCGTT
TTTCGAGCATGAATATGATTCGCTGAGTGAGGCCGATAAAGAGGTGTTTGTCCGCCTGCTGACCTGCGATGACCCCGATC
TGTTCAACTGGCTGATGAATCACGGCGAGCCGGACGATACCGAATTACAACGCATGGTCGCCCTTATTCAGACGCGAAAC
AAAGCCCGTGGCCCTGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References