Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 10117..10639 Replicon plasmid pBY63-3
Accession NZ_CP090573
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag LZE53_RS24490 Protein ID WP_000220560.1
Coordinates 10358..10639 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag LZE53_RS24485 Protein ID WP_000121743.1
Coordinates 10117..10368 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS24455 (LZE53_24365) 5480..5710 + 231 WP_000051064.1 plasmid partition protein ParG -
LZE53_RS24460 (LZE53_24370) 5726..6019 + 294 WP_015060510.1 hypothetical protein -
LZE53_RS24465 (LZE53_24375) 6021..6989 + 969 WP_077783213.1 IS5-like element IS903B family transposase -
LZE53_RS24470 (LZE53_24380) 7049..7402 - 354 WP_000876434.1 DNA distortion polypeptide 3 -
LZE53_RS24475 (LZE53_24385) 7539..7985 - 447 WP_001074386.1 hypothetical protein -
LZE53_RS24480 (LZE53_24390) 8025..8861 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
LZE53_RS24485 (LZE53_24395) 10117..10368 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
LZE53_RS24490 (LZE53_24400) 10358..10639 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LZE53_RS24495 (LZE53_24405) 10785..11108 + 324 WP_001181903.1 hypothetical protein -
LZE53_RS24500 (LZE53_24410) 11153..11398 + 246 WP_000356546.1 hypothetical protein -
LZE53_RS24505 (LZE53_24415) 11388..11603 + 216 WP_001180116.1 hypothetical protein -
LZE53_RS24510 (LZE53_24420) 11696..12025 + 330 WP_000866648.1 hypothetical protein -
LZE53_RS24515 (LZE53_24425) 12312..12461 + 150 WP_000003880.1 hypothetical protein -
LZE53_RS24520 (LZE53_24430) 12474..12746 + 273 WP_000160399.1 hypothetical protein -
LZE53_RS24525 (LZE53_24435) 13072..13617 + 546 WP_015060502.1 DNA distortion polypeptide 1 -
LZE53_RS24530 (LZE53_24440) 13620..14786 + 1167 WP_000539530.1 MobP1 family relaxase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..41043 41043
- flank IS/Tn - - 6066..6989 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T231253 WP_000220560.1 NZ_CP090573:10358-10639 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T231253 NZ_CP126167:58048-58353 [Salmonella enterica subsp. enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT231253 WP_000121743.1 NZ_CP090573:10117-10368 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT231253 NZ_CP126167:57828-58046 [Salmonella enterica subsp. enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References