Detailed information of TA system TA231250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 84533..85176
• Replicon: plasmid pBY63-1
• Accession: NZ_CP090571
• Organism: Escherichia coli strain BY63

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: LZE53_RS23770
• Protein ID: WP_000754566.1
• Coordinates: 84760..85176 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LZE53_RS23765
• Protein ID: WP_001261276.1
• Coordinates: 84533..84763 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZE53_RS23730 (LZE53_23640)	79683..80573	+	891	Protein_88	IS30-like element IS30 family transposase	-
LZE53_RS23735 (LZE53_23645)	80596..81624	-	1029	WP_063097364.1	tyrosine-type recombinase/integrase	-
LZE53_RS23740 (LZE53_23650)	82018..82493	+	476	Protein_90	IS1 family transposase	-
LZE53_RS23745 (LZE53_23655)	82770..82979	-	210	Protein_91	IS110 family transposase	-
LZE53_RS23750 (LZE53_23660)	83033..83737	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LZE53_RS23755 (LZE53_23665)	83843..84334	+	492	Protein_93	IS5-like element ISKpn26 family transposase	-
LZE53_RS23760 (LZE53_23670)	84322..84576	-	255	Protein_94	hypothetical protein	-
LZE53_RS23765 (LZE53_23675)	84533..84763	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LZE53_RS23770 (LZE53_23680)	84760..85176	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZE53_RS23775 (LZE53_23685)	85381..86237	+	857	Protein_97	IS3 family transposase	-
LZE53_RS23780 (LZE53_23690)	86809..87210	+	402	WP_096171525.1	hypothetical protein	-
LZE53_RS23785 (LZE53_23695)	87310..87867	-	558	WP_001235700.1	recombinase family protein	-
LZE53_RS23790 (LZE53_23700)	88031..88171	+	141	WP_020219413.1	hypothetical protein	-
LZE53_RS23795 (LZE53_23705)	88183..88887	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
LZE53_RS23800 (LZE53_23710)	88957..89157	+	201	WP_000993264.1	DUF3330 domain-containing protein	-
LZE53_RS23805 (LZE53_23715)	89283..89843	+	561	WP_000147567.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	floR / aac(3)-IId / tet(M) / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia	-	1..103545	103545
	-	inside	IScluster/Tn	-	-	76590..99573	22983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T231250 WP_000754566.1 NZ_CP090571:84760-85176 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T231250 NZ_CP126166:c4093423-4093268 [Salmonella enterica subsp. enterica]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT231250 WP_001261276.1 NZ_CP090571:84533-84763 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT231250 NZ_CP126166:4093435-4093493 [Salmonella enterica subsp. enterica]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023