Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3518980..3519598 Replicon chromosome
Accession NZ_CP090570
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LZE53_RS17210 Protein ID WP_001291435.1
Coordinates 3519380..3519598 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LZE53_RS17205 Protein ID WP_000344800.1
Coordinates 3518980..3519354 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS17195 (3514069) 3514069..3515262 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LZE53_RS17200 (3515285) 3515285..3518434 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LZE53_RS17205 (3518980) 3518980..3519354 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LZE53_RS17210 (3519380) 3519380..3519598 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LZE53_RS17215 (3519771) 3519771..3520322 + 552 WP_000102564.1 maltose O-acetyltransferase -
LZE53_RS17220 (3520438) 3520438..3520908 + 471 WP_000136192.1 YlaC family protein -
LZE53_RS17225 (3521072) 3521072..3522622 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LZE53_RS17230 (3522664) 3522664..3523017 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LZE53_RS17240 (3523396) 3523396..3523707 + 312 WP_000409911.1 MGMT family protein -
LZE53_RS17245 (3523738) 3523738..3524310 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T231246 WP_001291435.1 NZ_CP090570:3519380-3519598 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T231246 NZ_CP126166:1981147-1981422 [Salmonella enterica subsp. enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT231246 WP_000344800.1 NZ_CP090570:3518980-3519354 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT231246 NZ_CP126166:1980884-1981144 [Salmonella enterica subsp. enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References