Detailed information of TA system TA231245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2534247..2534885
• Replicon: chromosome
• Accession: NZ_CP090570
• Organism: Escherichia coli strain BY63

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LZE53_RS12515
• Protein ID: WP_000813794.1
• Coordinates: 2534709..2534885 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LZE53_RS12510
• Protein ID: WP_001270286.1
• Coordinates: 2534247..2534663 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZE53_RS12490 (2529399)	2529399..2530340	-	942	WP_001251304.1	ABC transporter permease	-
LZE53_RS12495 (2530341)	2530341..2531354	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LZE53_RS12500 (2531372)	2531372..2532517	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LZE53_RS12505 (2532762)	2532762..2534168	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LZE53_RS12510 (2534247)	2534247..2534663	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LZE53_RS12515 (2534709)	2534709..2534885	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LZE53_RS12520 (2535107)	2535107..2535337	+	231	WP_000494244.1	YncJ family protein	-
LZE53_RS12525 (2535429)	2535429..2537390	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LZE53_RS12530 (2537463)	2537463..2537999	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LZE53_RS12535 (2538052)	2538052..2539266	+	1215	WP_071790389.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T231245 WP_000813794.1 NZ_CP090570:c2534885-2534709 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T231245 NZ_CP126166:1932728-1933096 [Salmonella enterica subsp. enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTAGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT231245 WP_001270286.1 NZ_CP090570:c2534663-2534247 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT231245 NZ_CP126166:1932510-1932731 [Salmonella enterica subsp. enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• PDB: 6HPB