Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 922375..923029 Replicon chromosome
Accession NZ_CP090570
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LZE53_RS04540 Protein ID WP_000244777.1
Coordinates 922622..923029 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LZE53_RS04535 Protein ID WP_000354046.1
Coordinates 922375..922641 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS04515 (918344) 918344..919777 - 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -
LZE53_RS04520 (919822) 919822..920133 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
LZE53_RS04525 (920297) 920297..920956 + 660 WP_000250274.1 hemolysin III family protein -
LZE53_RS04530 (921152) 921152..922132 - 981 WP_001514522.1 tRNA-modifying protein YgfZ -
LZE53_RS04535 (922375) 922375..922641 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LZE53_RS04540 (922622) 922622..923029 + 408 WP_000244777.1 protein YgfX Toxin
LZE53_RS04545 (923069) 923069..923590 - 522 WP_001055874.1 flavodoxin FldB -
LZE53_RS04550 (923702) 923702..924598 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LZE53_RS04555 (924623) 924623..925333 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LZE53_RS04560 (925339) 925339..927072 + 1734 WP_032187585.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T231234 WP_000244777.1 NZ_CP090570:922622-923029 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T231234 NZ_CP126149:c39443-38805 [Sagittula sp. MA-2]
ATGGCCAACTTGACCGCTGAAAAAGCACTGATCTTCAGGATCACGCACATAAACAATGTGCCGTGGATCCTCAGGCATGG
GCTTCATTGCAAGAATTCCGGTGTGCAGGACCCCAATTTCGTACGAATTGGGAACCTGGAACTGATTCAGCGTCGGACGG
CACGGAATGTTCCTGTACCCCCGGGTGGAACGCTTTCAGACTACATACCCTTCTATTTCACGCCATTTTCGATGATGATG
TACAACATCAAGACTGGTTACGGAGGAATTCGTCAGTTCCTGAACGCGGAGATCGTGATCATGGCTTCGTCACTTCGTGG
GCTCGCGGATCGAGGCGTAGCGACAGTGTTCTCGGATCGTCATGCATACCTCCAGACCGCGCAGTTCTTTACCTCGCTCG
ACGACCTCGACAAAATCGACTGGGGCATCCTGCAGCGTAGGGACTTCAAACGCGATGTCGATGATCCCGAAAAGACAGAC
CGCTACCAGGCCGAGGCGCTGGTGCACAGGCACTTGCCAGTTGAGCACCTTGCAGGCATAGTCTGCCTGAGTGAGAACGA
AAAAGGAACACTCGAACGGCAACGGGAGGAGGTTGGGCTCGATCTCAAGATCGTGGCGCGACCGGGCTGGTATTTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT231234 WP_000354046.1 NZ_CP090570:922375-922641 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT231234 NZ_CP126149:c38805-37726 [Sagittula sp. MA-2]
ATGGTAACTTACACGCGGGGCAATCTTCTTGAGGCCGACGTCGACGCGGTGGTGAACACCGTGAACACGGTCGGAATCAT
GGGCAAAGGAATTGCCCTGATGTTCAAGGAGCAATTCCCAAGAAACTTCGAAGCCTATGCGCGCGCCTGCGATGCGGGCG
AAGTCCGTGTTGGCAAAATGTTCGTTACCGAGAACAAGGAACTCTTCGGGCCGCGCTGGATCATCAATTTCCCGACCAAG
ACCCATTGGCGAGTCAAAACCAAGATCGAATGGGTCGAAGACGGATTGAAGGATCTTGTCCGAGTAATTCGAGAGAAGGA
AATTCAATCAATTGCAATTCCGCCACTTGGCTGCGGGAATGGCGGCCTCGACTGGAGCGATGTGCGTCCACTTATCGAGG
CGTCATTGGCGGAACTTGATGGGGTCAATGTCATCGTCTACGAGCCGACTTCCAAGTATCAGAATGTAGCCAAGCGCACC
GGTGTTGAGAAGCTCACCCCAGCACGCGCATTGGTGGCTGAGATGGTGCGTCGGTACTCCCTTCTTGGGATCGAGTGCTC
GATTCTTGAGGTCCAGAAGCTGGGTTGGTTCCTCGAGCGCGGCGTGACGCGGTTCGGAGGATCGGATGCACTCAAGTTCA
GGTTCCAGGCGAACAAATACGGACCTTATTCGCATAACCTCACGAAGTTGCTCGATAGTCTCGACGGCAGTTATCTCCGG
TGCGACAAACGCCTCGCGGATGCCGATCCTATGGATCTCATTTGGTTCAACGACGCCAAATATGATCGGGTGCAAGCCTA
TCTGAACTCCGGTGAAGGCAAGCAGTTCTCAAGCGTATTGGAATGGGCTTCGGCCACCATTGATGGATTCGAGTCGCCAC
TCGGCATGGAACTTCTGGCGACTGTCGACTGGATGATGCAGCACGACGACATCGAACCGACTGTTGAGGGAGTGATGGAA
GGCCTTAAGGGCTGGGCTGGTGGGGAAGCCGCTGGACAGCGCAAATTGAAGATCTTCGATCGCCGATTGGTCGCCATCGC
CTTGGGCCAACTTCAGACATCGAACAGGCTGCCTGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References