Detailed information of TA system TA231212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 35648..36237
• Replicon: plasmid pCFBP2530-52
• Accession: NZ_CP090562
• Organism: Xanthomonas citri pv. malvacearum strain CFBP 2530

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LGM68_RS22800
• Protein ID: WP_078565930.1
• Coordinates: 35905..36237 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LGM68_RS22795
• Protein ID: WP_078565928.1
• Coordinates: 35648..35905 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM68_RS22780 (LGM68_22795)	31081..34158	+	3078	WP_269357717.1	type III secretion system effector avirulence protein AvrBs3	-
LGM68_RS22785 (LGM68_22800)	34273..34855	-	583	Protein_33	DUF4158 domain-containing protein	-
LGM68_RS22790 (LGM68_22805)	34839..35442	-	604	Protein_34	recombinase family protein	-
LGM68_RS22795 (LGM68_22810)	35648..35905	+	258	WP_078565928.1	antitoxin	Antitoxin
LGM68_RS22800 (LGM68_22815)	35905..36237	+	333	WP_078565930.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LGM68_RS22805	36514..36642	+	129	WP_269357720.1	hypothetical protein	-
LGM68_RS22810 (LGM68_22820)	36717..37253	+	537	WP_078565934.1	plasmid pRiA4b ORF-3 family protein	-
LGM68_RS22815 (LGM68_22825)	37987..38727	-	741	WP_269357723.1	hypothetical protein	-
LGM68_RS22820 (LGM68_22830)	38919..39185	-	267	WP_131830052.1	hypothetical protein	-
LGM68_RS22825 (LGM68_22835)	39670..40662	-	993	WP_269357725.1	hypothetical protein	-
LGM68_RS22830 (LGM68_22840)	40665..40844	-	180	WP_269357727.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51962	51962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11683.40 Da
• Isoelectric Point: 9.1975

>T231212 WP_078565930.1 NZ_CP090562:35905-36237 [Xanthomonas citri pv. malvacearum]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSSSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRGRKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T231212 NZ_CP126139:50276-50581 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9244.72 Da
• Isoelectric Point: 5.0214

>AT231212 WP_078565928.1 NZ_CP090562:35648-35905 [Xanthomonas citri pv. malvacearum]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPHYSLDELLVQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT231212 NZ_CP126139:50056-50274 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA