Detailed information of TA system TA231205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 23026..23597
• Replicon: plasmid unnamed1
• Accession: NZ_CP090559
• Organism: Enterococcus faecalis strain PCH555

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S4H3R9
• Locus tag: LZP99_RS13575
• Protein ID: WP_010784114.1
• Coordinates: 23256..23597 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S4FJJ0
• Locus tag: LZP99_RS13570
• Protein ID: WP_010784115.1
• Coordinates: 23026..23256 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZP99_RS13550	19442..19693	-	252	WP_002362426.1	hypothetical protein	-
LZP99_RS13905	19786..19914	+	129	WP_002409262.1	hypothetical protein	-
LZP99_RS13555	20320..20520	-	201	WP_002362428.1	hypothetical protein	-
LZP99_RS13560	21066..21791	-	726	WP_010784116.1	hypothetical protein	-
LZP99_RS13565	22202..22822	-	621	WP_023895233.1	recombinase family protein	-
LZP99_RS13570	23026..23256	+	231	WP_010784115.1	hypothetical protein	Antitoxin
LZP99_RS13575	23256..23597	+	342	WP_010784114.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LZP99_RS13580	23709..24647	+	939	WP_002362433.1	hypothetical protein	-
LZP99_RS13585	24912..25514	+	603	WP_002362434.1	Fic family protein	-
LZP99_RS13590	25583..26719	+	1137	WP_234746813.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ClpL	-	1..77536	77536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13270.47 Da
• Isoelectric Point: 7.9750

>T231205 WP_010784114.1 NZ_CP090559:23256-23597 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T231205 NZ_CP126138:c4105530-4105243 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8495.40 Da
• Isoelectric Point: 4.2404

>AT231205 WP_010784115.1 NZ_CP090559:23026-23256 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPILQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT231205 NZ_CP126138:c4105256-4104954 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: S4H3R9

Antitoxin

• AlphaFold DB: S4FJJ0