Detailed information of TA system TA231200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2714003..2714574
• Replicon: chromosome
• Accession: NZ_CP090558
• Organism: Enterococcus faecalis strain PCH555

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LZP99_RS12975
• Protein ID: WP_174094311.1
• Coordinates: 2714003..2714344 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LZP99_RS12980
• Protein ID: WP_002354773.1
• Coordinates: 2714344..2714574 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZP99_RS12970 (2710018)	2710018..2713632	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LZP99_RS12975 (2714003)	2714003..2714344	-	342	WP_174094311.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LZP99_RS12980 (2714344)	2714344..2714574	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LZP99_RS12985 (2714981)	2714981..2715196	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LZP99_RS12990 (2715335)	2715335..2716327	+	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LZP99_RS12995 (2716394)	2716394..2717026	-	633	WP_002358972.1	RloB family protein	-
LZP99_RS13000 (2717035)	2717035..2718330	-	1296	WP_104866226.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13255.54 Da
• Isoelectric Point: 9.7084

>T231200 WP_174094311.1 NZ_CP090558:c2714344-2714003 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQRQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T231200 NZ_CP126138:c996132-995605 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT231200 WP_002354773.1 NZ_CP090558:c2714574-2714344 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT231200 NZ_CP126138:c996419-996129 [Salmonella enterica subsp. enterica serovar Kottbus]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812