Detailed information of TA system TA231190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 137744..138763
• Replicon: chromosome
• Accession: NZ_CP090558
• Organism: Enterococcus faecalis strain PCH555

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: Q839N5
• Locus tag: LZP99_RS00595
• Protein ID: WP_002359283.1
• Coordinates: 137744..138382 (-)
• Length: 213 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LZP99_RS00600
• Protein ID: WP_002359284.1
• Coordinates: 138389..138763 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZP99_RS00585 (135559)	135559..136395	+	837	Protein_117	transposase	-
LZP99_RS00590 (137127)	137127..137702	+	576	WP_002359282.1	restriction endonuclease	-
LZP99_RS00595 (137744)	137744..138382	-	639	WP_002359283.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LZP99_RS00600 (138389)	138389..138763	-	375	WP_002359284.1	helix-turn-helix domain-containing protein	Antitoxin
LZP99_RS00605 (139605)	139605..139787	-	183	WP_002359286.1	hypothetical protein	-
LZP99_RS00610 (139954)	139954..140205	+	252	WP_002359287.1	hypothetical protein	-
LZP99_RS00615 (140292)	140292..140618	+	327	WP_002359288.1	hypothetical protein	-
LZP99_RS00620 (140618)	140618..141025	+	408	WP_002359289.1	DUF961 family protein	-
LZP99_RS00625 (141429)	141429..141620	+	192	WP_010773668.1	hypothetical protein	-
LZP99_RS00630 (141602)	141602..142006	+	405	WP_002359292.1	hypothetical protein	-
LZP99_RS00635 (142118)	142118..142450	+	333	WP_002359293.1	nucleotidyltransferase domain-containing protein	-
LZP99_RS00640 (142440)	142440..142823	+	384	WP_002359294.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	123582..183722	60140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (86-176)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 213 a.a.
• Molecular weight: 25186.21 Da
• Isoelectric Point: 9.1676

>T231190 WP_002359283.1 NZ_CP090558:c138382-137744 [Enterococcus faecalis]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDMPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILLK

Nucleotide

• Length: 639 bp

>T231190 NZ_CP126126:4010824-4011576 [Pseudomonas sp. SO81]
ATGGATATCTGGCAGGCCTGTGCCGGCGCAGAACAGCTGACTCCATTGCGCGGCCGGCTGGTACGCCTGGTGGAGAACCA
GGAGCAGGTGGCCACCCTGCAGCTGGTGGATACCCTGGAAGAGCAGGCGCTGCTCGAGCAGTTGCTGGAGGGCAGCAAGC
CGCCGCTACCACCCAGCGACGAGCCATTGCACTACTTGTTGCACACGCCGTTCCGTTACCCGCCGCTGCGTTGGGGGTCG
CGCTTCGGCCGGCGTCATGAGCCGAGCCTGTTCTATGCCGCCTGCCAGCTGGAAACGGCGATGGCCGAGACCGCCTTCTA
CCGTTTCGTGCTGTGGTACGGCATGGCCGAGCCGCCGCCCAGCGGGCGCATCCTCTCCGAGCATTCTTCCTTCGAGGCGC
GCTATCAGGTGCAGCGCGGCATTCGCCTGCAGCTGCCGCCGTTCGATCGGTACCGCCAGGCGCTGACTCATCGCAGCGAC
TACCGGGCTACCCATGAACTGGGCAGCGCCATGCGCGAGGCGGGGGTGGAGGCGTTCGAGTATCCCTCGGCGCGCTGCCC
ATTGCGGGGCACCAATGTCGCCCTGTATGCACCTGCCGCGTTCAGCGAGAAACGTCCACGCAACCTGCAGCCCTGGTTGT
GCGAAACCACCTCCGGATATGTGGCCTTCAAGAGTGCCCAGACGCCGGATATTCCCCGCCTGTTCGGCCTAGAGCTGTAC
CTGGTCGAGGGGCACCTGCCGCAGCCTGCCTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14518.29 Da
• Isoelectric Point: 4.8896

>AT231190 WP_002359284.1 NZ_CP090558:c138763-138389 [Enterococcus faecalis]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE

Nucleotide

• Length: 375 bp

>AT231190 NZ_CP126126:4010404-4010775 [Pseudomonas sp. SO81]
ATGAGTGCGCTCAGCAAGCCCGGCGTCGATGCCGATGCGGTATTGCTCAAGGCCCTGCTCAGTACCCGCGAGCAGTTGGG
GCTGACCCAGCAGGAATTGGCGGGAATAGTCGGAGTCAACCGTTCTGCCATCAGCCGCTGGGGCGATAGCGGCGGTCTGC
GTCCGCAGAGCAAGACTGGCGAACTGGCGCTGCTGCTGATCCGTATCTACCGCGCCCTGTTTGCCCTGTTTGGCGGCAAT
CTTGATGACATGCGCCATTTCCTGCGTACCGAGAACCGCCATCTGGCCGGTGTGCCGCTGGAGCAGATGGGCCAGGTGCA
GGGGCTGGTGCGTGTGGTGGAGTACCTCGACGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A059N6D0