Detailed information of TA system TA231186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-couple_hipB
• Location: 982873..983515
• Replicon: chromosome
• Accession: NZ_CP090556
• Organism: Actinobacillus suis strain D20_277

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0GF68
• Locus tag: L0F67_RS04955
• Protein ID: WP_005621659.1
• Coordinates: 982873..983244 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: L0F67_RS04960
• Protein ID: WP_005621656.1
• Coordinates: 983225..983515 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F67_RS04940 (L0F67_04925)	979378..980232	+	855	WP_015674480.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
L0F67_RS04945 (L0F67_04930)	980276..980845	-	570	WP_005608594.1	elongation factor P hydroxylase	-
L0F67_RS04950 (L0F67_04935)	980904..982655	-	1752	WP_015674481.1	protein-disulfide reductase DsbD	-
L0F67_RS04955 (L0F67_04940)	982873..983244	+	372	WP_005621659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0F67_RS04960 (L0F67_04945)	983225..983515	+	291	WP_005621656.1	helix-turn-helix transcriptional regulator	Antitoxin
L0F67_RS04965 (L0F67_04950)	983583..984544	-	962	Protein_937	calcium/sodium antiporter	-
L0F67_RS04970 (L0F67_04955)	984617..985114	+	498	WP_015674483.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
L0F67_RS04975 (L0F67_04960)	985342..986075	+	734	Protein_939	arginine ABC transporter ATP-binding protein ArtP	-
L0F67_RS04980 (L0F67_04965)	986095..986814	+	720	WP_015674485.1	transporter substrate-binding domain-containing protein	-
L0F67_RS04985 (L0F67_04970)	986820..987491	+	672	WP_015674486.1	arginine ABC transporter permease ArtQ	-
L0F67_RS04990 (L0F67_04975)	987491..988174	+	684	WP_015674487.1	arginine ABC transporter permease ArtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (33-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14938.10 Da
• Isoelectric Point: 9.5717

>T231186 WP_005621659.1 NZ_CP090556:982873-983244 [Actinobacillus suis]
MYEIVFYRDRRGREPVKEFLQEFINEQQDENRERLHKISHHLTILHLHGTRAGESYIKHLEDRIWQLRPISDCLLFAGIV
RGQFVLLHHFAKSSSRLPKRELERAKSRLADLQERIKDEPHWF

Nucleotide

• Length: 372 bp

>T231186 NZ_CP126113:c2915437-2915111 [Siminovitchia fortis]
ATGTCAGTACCAGACCGTGGGGATCTTGTTTATGTGAACTTTAATCCGCAGACCGGCCATGAGCAAGCGGGAAACAGGCC
AGGGATTGTTTTATCCCCAAAAGAGTTTAATCAAGTCACAGGATTTGCTTCCATCTGTCTTATCACAAAAACAGCCAGAG
GCTGGGGATTTGAAGTCAAGCTCCCCGAGGGGTTGGCTTTTCAAGGTGTGATCTTAACCGATCAGGTAAAAAATTTAGAT
TGGGCTGCGCGGAATCTTAAGGTTAAAGGAAAAGCGCCCGATGAATTGGTGGAGGAATGTTTGGCCAAAATACATACTTT
TTTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10803.58 Da
• Isoelectric Point: 8.7274

>AT231186 WP_005621656.1 NZ_CP090556:983225-983515 [Actinobacillus suis]
MSPIGSSWTSFEREVFNELEMEENHFRVKLILEIIETRQALGISQRKLEKLSGVKQSMIARVEKGSSNPSLGTLLKLLVP
LGKTLQIVPLDDTHSK

Nucleotide

• Length: 291 bp

>AT231186 NZ_CP126113:c2915730-2915437 [Siminovitchia fortis]
ATGGAAGAAGACACAAGGAGTAGTGGAAATGAGGTGGATCATATGACAATTCCAACCACAGTTCAAAAATGGGGCAATAG
CCTGGCGGTAAGAATTCCAAAGGATGTTGCAGAAAGAATTAACATTCATCAAGGGTCTGAAATGGAAATAAGCGTAACTG
GAAAAGGAGTTATCATGCTTGTTCCTAAAAAGCAGCCTAAGAAATATTCGCTGGAGGAACTCCTGGCAAAATGCACCCCG
GAAAACCGTCACGAGGAAATTGATTTTGGAGTAGAGGGGAAGGAGCTTATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A7MI46