Detailed information of TA system TA231184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 547172..548829
• Replicon: chromosome
• Accession: NZ_CP090556
• Organism: Actinobacillus suis strain D20_277

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: K0FZC7
• Locus tag: L0F67_RS02765
• Protein ID: WP_015674102.1
• Coordinates: 547498..548829 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: K0G670
• Locus tag: L0F67_RS02760
• Protein ID: WP_015674101.1
• Coordinates: 547172..547501 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F67_RS02745 (L0F67_02745)	543561..544841	+	1281	WP_015674098.1	threonine synthase	-
L0F67_RS02750 (L0F67_02750)	544919..545257	+	339	WP_015674099.1	hypothetical protein	-
L0F67_RS02755 (L0F67_02755)	545554..546878	-	1325	Protein_521	MFS transporter family glucose-6-phosphate receptor UhpC	-
L0F67_RS02760 (L0F67_02760)	547172..547501	-	330	WP_015674101.1	helix-turn-helix domain-containing protein	Antitoxin
L0F67_RS02765 (L0F67_02765)	547498..548829	-	1332	WP_015674102.1	HipA domain-containing protein	Toxin
L0F67_RS02770 (L0F67_02770)	549223..550251	+	1029	Protein_524	Abi family protein	-
L0F67_RS02775 (L0F67_02775)	550408..551445	-	1038	WP_015674105.1	YiiX/YebB-like N1pC/P60 family cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51155.04 Da
• Isoelectric Point: 5.1273

>T231184 WP_015674102.1 NZ_CP090556:c548829-547498 [Actinobacillus suis]
MSDISIKIKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSISINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAIVGG
ATGAGGEAPKLLLKRQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNVISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNSQEFAGEYRFDKIADSLSDLIEPDILLRELR
MLATQLVDLKSRLTKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T231184 NZ_CP126112:280129-280479 [Peribacillus simplex]
ATTGTTGTTAAGCGTGGTGACGTATACTTCGCAGACCTTTCCCCGGTGGTTGGTTCAGAGCAGGGTGGGACCCGTCCGGT
ACTAATTTTACAAAACGATATTGGTAATCGCTTTAGTCCGACTGTGATCGTGGCAGCCATTACAGCTCAAATTCAAAAAG
CGAAATTGCCTACACATGTTGAGATTAATGCAAAAAAATACGGATTCGAGCGAGATTCCGTCATTTTATTAGAACAGATC
CGGACAATTGATAAGCAGCGTTTAACTGATAAAATTACGCATCTTGACGAACCGATGATGCAAAAAGTCAATGAAGCTTT
GCAAATTAGTTTAGGCCTCATTGAGTTTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12364.46 Da
• Isoelectric Point: 10.6799

>AT231184 WP_015674101.1 NZ_CP090556:c547501-547172 [Actinobacillus suis]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMDSFLK

Nucleotide

• Length: 330 bp

>AT231184 NZ_CP126112:279840-280121 [Peribacillus simplex]
GTGTCTGAATCCAGCGCAACAAGAGAGATATTAATTCGATTACCTCAAAATTTTTTAACAGAGTTAGATGGGTATGCGAG
TGAGGAAAATGTGAATCGCAGTGAATTTATTTATCGTGCCACAAAAATGTATCTTCGCGAACGTAAAAAGAAAGAGTTTC
GTGAATCGATGAAACGCGGTTATATTGAAATGGCGGCCATTAATTTAACGATTGCTTCTGAAGCCTTCCAGGCTGAATTC
GAGGCTGGTCATTGCGTTGAACGATTAGTTAGCGGAGGCTGA

Structures

Toxin

• AlphaFold DB: K0FZC7

Antitoxin

• AlphaFold DB: K0G670