Detailed information of TA system TA231183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 485264..485995
• Replicon: chromosome
• Accession: NZ_CP090556
• Organism: Actinobacillus suis strain D20_277

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: K0FZ70
• Locus tag: L0F67_RS02400
• Protein ID: WP_015674046.1
• Coordinates: 485264..485731 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: K0G6P2
• Locus tag: L0F67_RS02405
• Protein ID: WP_015674047.1
• Coordinates: 485735..485995 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F67_RS02385 (L0F67_02385)	481206..482582	+	1377	WP_015674043.1	Trk system potassium transporter TrkA	-
L0F67_RS02390 (L0F67_02390)	482795..484189	+	1395	WP_015674044.1	class II fumarate hydratase	-
L0F67_RS02395 (L0F67_02395)	484300..485181	-	882	WP_015674045.1	50S ribosomal protein L11 methyltransferase	-
L0F67_RS02400 (L0F67_02400)	485264..485731	-	468	WP_015674046.1	GNAT family N-acetyltransferase	Toxin
L0F67_RS02405 (L0F67_02405)	485735..485995	-	261	WP_015674047.1	DUF1778 domain-containing protein	Antitoxin
L0F67_RS02410 (L0F67_02410)	486116..487576	-	1461	WP_015674048.1	metalloprotease TldD	-
L0F67_RS02415 (L0F67_02415)	487706..488965	+	1260	WP_015674049.1	tRNA lysidine(34) synthetase TilS	-
L0F67_RS02420 (L0F67_02420)	489066..489566	-	501	WP_015674050.1	thiol peroxidase	-
L0F67_RS02425 (L0F67_02425)	489699..490422	+	724	Protein_461	1-acylglycerol-3-phosphate O-acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17157.14 Da
• Isoelectric Point: 8.9515

>T231183 WP_015674046.1 NZ_CP090556:c485731-485264 [Actinobacillus suis]
MHAPELLSEQHIVQHFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSRQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T231183 NZ_CP126110:402380-402730 [Neobacillus sp. WH10]
TTGATTGTCAAACGTGGTGACGTATATTTCGCGGACCTATCCCCAGTTGTTGGTTCTGAACAAGGCGGTGTCCGACCTGT
ACTTGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCGATTACAGCTCAAATTCAGAAAG
CGAAGCTTCCTACTCATGTTGAAATTGATGCTAAACGCTACGGATTTGAACGAGACTCGGTCATTTTGTTAGAGCAAATT
CGTACAATTGATAAGCAGCGGTTAACCGATAAAATTACCCATCTCGATGACGAAATGATGGAGAAAGTGGATGAAGCCTT
GCAGGTAAGTTTAGGTCTCATCGAATTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9826.17 Da
• Isoelectric Point: 4.4883

>AT231183 WP_015674047.1 NZ_CP090556:c485995-485735 [Actinobacillus suis]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQTFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT231183 NZ_CP126110:402094-402375 [Neobacillus sp. WH10]
GTGTCTGAATCCGGCGCAACTACGGAAATCTTAGTAAAGTTACCGCAACATCTTTTAACTGAGTTAGATGGTTTCGTTAA
ACAAGAAAATGTAAACCGCAGCGAGTTTATTTACCAAGCAACAAAAATGTATTTGCGCGAACGAAAAAAGAGACAAATTC
GCGAGTCGATGAGACGTGGATACATGGAAATGGCAAAGATAAATCTTAGAATTGCTTCTGAAGCATTTCAAGCAGAATAT
GAGGCAGAACATACTGTAGAACGTCTTGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: K0FZ70

Antitoxin

• AlphaFold DB: K0G6P2