Detailed information of TA system TA231182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 27567..28237
• Replicon: plasmid pXF30-OC8
• Accession: NZ_CP090548
• Organism: Xylella fastidiosa subsp. sandyi strain OC8

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: LZ756_RS13280
• Protein ID: WP_020852254.1
• Coordinates: 27567..27917 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: LZ756_RS13285
• Protein ID: WP_020852255.1
• Coordinates: 27914..28237 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ756_RS13250 (LZ756_13250)	23151..23603	-	453	WP_051404191.1	type II toxin-antitoxin system VapC family toxin	-
LZ756_RS13255 (LZ756_13255)	23603..23857	-	255	WP_024748605.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LZ756_RS13260 (LZ756_13260)	24215..24364	+	150	WP_230577912.1	recombinase family protein	-
LZ756_RS13265 (LZ756_13265)	24825..26321	+	1497	WP_020852252.1	D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase	-
LZ756_RS13270 (LZ756_13270)	26894..27070	+	177	WP_196230051.1	hypothetical protein	-
LZ756_RS13275 (LZ756_13275)	27074..27494	+	421	Protein_33	virulence factor	-
LZ756_RS13280 (LZ756_13280)	27567..27917	+	351	WP_020852254.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ756_RS13285 (LZ756_13285)	27914..28237	+	324	WP_020852255.1	helix-turn-helix transcriptional regulator	Antitoxin
LZ756_RS13290 (LZ756_13290)	28277..28496	+	220	Protein_36	GNAT family N-acetyltransferase	-
LZ756_RS13295 (LZ756_13295)	28971..29150	+	180	WP_014607310.1	hypothetical protein	-
LZ756_RS13300 (LZ756_13300)	29153..29632	+	480	WP_020852256.1	hypothetical protein	-
LZ756_RS13305 (LZ756_13305)	29635..29955	+	321	WP_020852257.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30305	30305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12745.60 Da
• Isoelectric Point: 8.8688

>T231182 WP_020852254.1 NZ_CP090548:27567-27917 [Xylella fastidiosa subsp. sandyi]
MKPLVWIASAKKDLMAMPDDVQDIFGYALHLAQTGEKHDQAKSLKGFGSAGVLEVVEDSDGGTYRGVYTVRFGNAVYVLH
CFQKKSTHGIATSKPDIDLIRDRLKVAEAHAKGERK

Nucleotide

• Length: 351 bp

>T231182 NZ_CP126109:218328-218678 [Fictibacillus enclensis]
TTGATCGTAAAACGCGGAGACGTTTACTTTGCAGACCTTTCCCCGGTTGTTGGATCAGAACAAGGGGGAATCAGACCTGT
TCTAATTATTCAAAATGATATCGGTAACCGATTTAGTCCTACAGTTATTGTTGCAGCCATTACAGCACAAATCCAAAAGG
CGAAGCTGCCGACGCATGTAGAGATCAATGCGAAAAAGTACGGATTTGAACGGGATTCTGTTATCTTGCTGGAGCAAATT
CGAACAATTGATAAACAAAGGCTAACTGACAAAATTACGCATCTGGACGATGAAATGATGCGTCGCGTTGATGAAGCGTT
ACAAATCAGTCTTGGTCTTATTGACTTTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11635.52 Da
• Isoelectric Point: 5.8625

>AT231182 WP_020852255.1 NZ_CP090548:27914-28237 [Xylella fastidiosa subsp. sandyi]
MIEIEEGSGNVYADLGIPDADEMLVKAQLATKIGEIIKGRGWTQQEAADVLGMTQPKLSKMLRGQFRGISEAKMLECLAR
LGRQVQIVVGPARRTNDVGHVEVIFAA

Nucleotide

• Length: 324 bp

>AT231182 NZ_CP126109:218042-218323 [Fictibacillus enclensis]
GTGTCCGAATTGGCGAACACAAAGAGGATCGTTATCAGTCTTCCTCAACAGATTATCAATGAAATTGACCGGATGATTAA
GAATGAGAATTTGGACCGTAGCGAATTTATCCATCAGGCAACCAAGATGTATATTCGCGAAAAGAAGAAACGTCACATTC
GTGAATCTATGAGACAAGGTTATATGGAAATGGCGAAAATTAATCTGAACATTGCATCAGAAGCCTTTCTTTTAGAGGAG
GAAGCTGAAATTACCGTAGACCGCTTAGTTAGCGGGGTGTAG