Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/PRK09812-MazE |
| Location | 13110..13696 | Replicon | plasmid pXF30-OC8 |
| Accession | NZ_CP090548 | ||
| Organism | Xylella fastidiosa subsp. sandyi strain OC8 | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | A0A060HFH6 |
| Locus tag | LZ756_RS13170 | Protein ID | WP_024748613.1 |
| Coordinates | 13110..13436 (-) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | D5LL99 |
| Locus tag | LZ756_RS13175 | Protein ID | WP_013087943.1 |
| Coordinates | 13433..13696 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LZ756_RS13150 (LZ756_13150) | 8178..10421 | + | 2244 | WP_234759440.1 | type IV secretory system conjugative DNA transfer family protein | - |
| LZ756_RS13155 (LZ756_13155) | 10434..11540 | + | 1107 | WP_020852265.1 | hypothetical protein | - |
| LZ756_RS13160 (LZ756_13160) | 11588..11875 | + | 288 | WP_020852266.1 | DUF4190 domain-containing protein | - |
| LZ756_RS13165 (LZ756_13165) | 12089..13051 | + | 963 | WP_230577909.1 | relaxase/mobilization nuclease domain-containing protein | - |
| LZ756_RS13170 (LZ756_13170) | 13110..13436 | - | 327 | WP_024748613.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LZ756_RS13175 (LZ756_13175) | 13433..13696 | - | 264 | WP_013087943.1 | antitoxin | Antitoxin |
| LZ756_RS13180 (LZ756_13180) | 13808..15016 | + | 1209 | WP_042836803.1 | RNA-guided endonuclease TnpB family protein | - |
| LZ756_RS13185 (LZ756_13185) | 15165..15311 | - | 147 | WP_230577911.1 | hypothetical protein | - |
| LZ756_RS13190 (LZ756_13190) | 15330..15959 | - | 630 | WP_230577910.1 | plasmid mobilization relaxosome protein MobC | - |
| LZ756_RS13195 (LZ756_13195) | 16333..16593 | + | 261 | WP_199276035.1 | hypothetical protein | - |
| LZ756_RS13200 (LZ756_13200) | 16606..17358 | + | 753 | WP_020852243.1 | hypothetical protein | - |
| LZ756_RS13205 (LZ756_13205) | 17359..17802 | + | 444 | WP_014607318.1 | hypothetical protein | - |
| LZ756_RS13210 (LZ756_13210) | 17933..18262 | - | 330 | WP_024748578.1 | type II toxin-antitoxin system YafQ family toxin | - |
| LZ756_RS13215 (LZ756_13215) | 18234..18512 | - | 279 | WP_024748579.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..30305 | 30305 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11795.71 Da Isoelectric Point: 8.5480
>T231181 WP_024748613.1 NZ_CP090548:c13436-13110 [Xylella fastidiosa subsp. sandyi]
MKRGDVYMVDLEPTAGHEQRGHRPVVVISSERFNRLTSCPVILPITNGGEFASRLGFAVELLGTITTGVVRCDQPRALDL
IARNARKVESLPPNILADVLAKAVTIFQ
MKRGDVYMVDLEPTAGHEQRGHRPVVVISSERFNRLTSCPVILPITNGGEFASRLGFAVELLGTITTGVVRCDQPRALDL
IARNARKVESLPPNILADVLAKAVTIFQ
Download Length: 327 bp
>T231181 NZ_CP126108:295517-295915 [Cytobacillus firmus]
TTGAGGGATATAGAGGCTAAAACTGGACGAAAGGTTAATAAAGAGCAAATTGATCTAATAAAAGAAGACTTGAGAAACAA
AAACTATGAAAAATTGACACCAAGAGAGACAGCTAAGCATAGAAGCAAATTTACATCGAGCTTGAAAGATAAATTGATTG
CAGAATGGGAAGAAAAAACTAATCAAAAGTGGCCAAGATATACAGAAGAAGTGCTTGATAAGAATGGAGAGGTTGCTCGG
AGTATTGGTCAACCGTATGATGCCCATCATATCATAGAGAACAATTTTGGCGGCCCTCATGAGTGGTGGAATATTCATCC
CGCAAAATATCCTAATGAACACCAAGCAGGGATACATGGAAAGGGAGCTCCATCGGGTAAACTATTTCCAAGGAGGTAA
TTGAGGGATATAGAGGCTAAAACTGGACGAAAGGTTAATAAAGAGCAAATTGATCTAATAAAAGAAGACTTGAGAAACAA
AAACTATGAAAAATTGACACCAAGAGAGACAGCTAAGCATAGAAGCAAATTTACATCGAGCTTGAAAGATAAATTGATTG
CAGAATGGGAAGAAAAAACTAATCAAAAGTGGCCAAGATATACAGAAGAAGTGCTTGATAAGAATGGAGAGGTTGCTCGG
AGTATTGGTCAACCGTATGATGCCCATCATATCATAGAGAACAATTTTGGCGGCCCTCATGAGTGGTGGAATATTCATCC
CGCAAAATATCCTAATGAACACCAAGCAGGGATACATGGAAAGGGAGCTCCATCGGGTAAACTATTTCCAAGGAGGTAA
Antitoxin
Download Length: 88 a.a. Molecular weight: 9365.77 Da Isoelectric Point: 4.3588
>AT231181 WP_013087943.1 NZ_CP090548:c13696-13433 [Xylella fastidiosa subsp. sandyi]
MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGATVGLAVTDGRLVIEPVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAP
AVGRELI
MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGATVGLAVTDGRLVIEPVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAP
AVGRELI
Download Length: 264 bp
>AT231181 NZ_CP126108:295924-296361 [Cytobacillus firmus]
ATGGCTGATTTTGAATTTATAAATGAACTAGAAAATAAACTTTATAAGGTAACTAATGATGATATATTAAAAGCTGAACA
AAGAATGGACATCAGCCTTCCAAATGATTTAAAACAGTTATATTTAGAAGTTGGTTATGGTTTTATTGAAGGTCTAAGTG
CTAATTCAATTAATAGAATATTGGGACCAGGGGCTGTAGCAGATATTAGATTAAGGGAAGGTGTTTTTGAATTTGATCCA
GATTTAGATGAACTTTTTGATGATGAAGATAAACTTATTTTTTTTGAAGTAAACGAGGGTGTTTATATTTCGATAGATTT
ACAATTAGCTAATAATCCAATATTTTATTTCGATACTCAAATAGCTGAATCGTTAGAGGATTTTTTTAAAAAGTTTCTCA
ATAATAATGAATATTTTATAGATTTAATTGAGGATTAG
ATGGCTGATTTTGAATTTATAAATGAACTAGAAAATAAACTTTATAAGGTAACTAATGATGATATATTAAAAGCTGAACA
AAGAATGGACATCAGCCTTCCAAATGATTTAAAACAGTTATATTTAGAAGTTGGTTATGGTTTTATTGAAGGTCTAAGTG
CTAATTCAATTAATAGAATATTGGGACCAGGGGCTGTAGCAGATATTAGATTAAGGGAAGGTGTTTTTGAATTTGATCCA
GATTTAGATGAACTTTTTGATGATGAAGATAAACTTATTTTTTTTGAAGTAAACGAGGGTGTTTATATTTCGATAGATTT
ACAATTAGCTAATAATCCAATATTTTATTTCGATACTCAAATAGCTGAATCGTTAGAGGATTTTTTTAAAAAGTTTCTCA
ATAATAATGAATATTTTATAGATTTAATTGAGGATTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A060HFH6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A060H856 |