Detailed information of TA system TA231172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1519540..1520213
• Replicon: chromosome
• Accession: NZ_CP090547
• Organism: Xylella fastidiosa subsp. sandyi strain OC8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LZ756_RS07305
• Protein ID: WP_020851658.1
• Coordinates: 1519540..1519932 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LZ756_RS07310
• Protein ID: WP_024748527.1
• Coordinates: 1519941..1520213 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ756_RS07275 (LZ756_07275)	1514597..1515130	+	534	WP_020852388.1	hypothetical protein	-
LZ756_RS07280 (LZ756_07280)	1515131..1516626	+	1496	Protein_1417	DUF3383 domain-containing protein	-
LZ756_RS07285 (LZ756_07285)	1516636..1517073	+	438	WP_004087249.1	DUF3277 family protein	-
LZ756_RS07290 (LZ756_07290)	1517070..1517492	+	423	WP_020851660.1	putative phage tail assembly chaperone	-
LZ756_RS07295 (LZ756_07295)	1517519..1517683	+	165	WP_162849007.1	hypothetical protein	-
LZ756_RS07300 (LZ756_07300)	1517640..1519529	+	1890	WP_020851659.1	phage-related protein	-
LZ756_RS07305 (LZ756_07305)	1519540..1519932	-	393	WP_020851658.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZ756_RS07310 (LZ756_07310)	1519941..1520213	-	273	WP_024748527.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LZ756_RS07315 (LZ756_07315)	1520373..1521143	+	771	WP_020851656.1	hypothetical protein	-
LZ756_RS07320 (LZ756_07320)	1521143..1521460	+	318	WP_020851655.1	hypothetical protein	-
LZ756_RS07325 (LZ756_07325)	1521457..1522287	+	831	WP_020851654.1	hypothetical protein	-
LZ756_RS07330 (LZ756_07330)	1522370..1522924	+	555	WP_234759298.1	Gp138 family membrane-puncturing spike protein	-
LZ756_RS07335 (LZ756_07335)	1522921..1523274	+	354	WP_020851652.1	hypothetical protein	-
LZ756_RS07340 (LZ756_07340)	1523285..1523590	-	306	WP_004089254.1	putative addiction module antidote protein	-
LZ756_RS07345 (LZ756_07345)	1523594..1523890	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ756_RS07350 (LZ756_07350)	1523989..1525134	+	1146	WP_020851651.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1434171..1526858	92687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• SpoVT-AbrB domain (16-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14247.51 Da
• Isoelectric Point: 5.6881

>T231172 WP_020851658.1 NZ_CP090547:c1519932-1519540 [Xylella fastidiosa subsp. sandyi]
MLDTNIISDIIRNPFGAVACRIEHVGYPNICTSVIVAAELRYGCTKNGSVKLLSRVQDILKTLPILPLDIPVDTTYGSIR
AELEAAGQLIGANDLLIAAHAYVLGLTLVTDNTREFSRIRGLDVQNWLER

Nucleotide

• Length: 393 bp

>T231172 NZ_CP126100:c2252982-2252806 [Bacillus halotolerans]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10396.87 Da
• Isoelectric Point: 4.8782

>AT231172 WP_024748527.1 NZ_CP090547:c1520213-1519941 [Xylella fastidiosa subsp. sandyi]
MPISSPLSRTKEAKLFRNNRSQAVRIPVEFELSGDRVLIHREGSKLIIEPITRPTNIVELLHEWRRETPLGPEDQFPDID
DMPAQPEDIL

Nucleotide

• Length: 273 bp

>AT231172 NZ_CP126100:2252923-2253020 [Bacillus halotolerans]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTT
TGACTCTATTATAACATA