Detailed information of TA system TA231170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1060155..1060760
• Replicon: chromosome
• Accession: NZ_CP090547
• Organism: Xylella fastidiosa subsp. sandyi strain OC8

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: LZ756_RS04865
• Protein ID: WP_004089252.1
• Coordinates: 1060464..1060760 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LZ756_RS04860
• Protein ID: WP_004089254.1
• Coordinates: 1060155..1060460 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ756_RS04825 (LZ756_04825)	1056409..1056801	-	393	WP_020851658.1	type II toxin-antitoxin system VapC family toxin	-
LZ756_RS04830 (LZ756_04830)	1056810..1057082	-	273	WP_024748527.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LZ756_RS04835 (LZ756_04835)	1057242..1058012	+	771	WP_020851656.1	hypothetical protein	-
LZ756_RS04840 (LZ756_04840)	1058012..1058329	+	318	WP_020851655.1	hypothetical protein	-
LZ756_RS04845 (LZ756_04845)	1058326..1059156	+	831	WP_020851654.1	hypothetical protein	-
LZ756_RS04850 (LZ756_04850)	1059153..1059794	+	642	WP_020851653.1	Gp138 family membrane-puncturing spike protein	-
LZ756_RS04855 (LZ756_04855)	1059791..1060144	+	354	WP_020851652.1	hypothetical protein	-
LZ756_RS04860 (LZ756_04860)	1060155..1060460	-	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
LZ756_RS04865 (LZ756_04865)	1060464..1060760	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ756_RS04870 (LZ756_04870)	1060859..1062004	+	1146	WP_020851651.1	baseplate J/gp47 family protein	-
LZ756_RS04875 (LZ756_04875)	1062001..1062561	+	561	WP_020851650.1	DUF2612 domain-containing protein	-
LZ756_RS04880 (LZ756_04880)	1062565..1063809	+	1245	WP_024748982.1	tail fiber protein	-
LZ756_RS04885 (LZ756_04885)	1063913..1064359	-	447	WP_020852919.1	hypothetical protein	-
LZ756_RS04890 (LZ756_04890)	1064618..1065586	-	969	WP_020852918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pilT	1021848..1322833	300985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T231170 WP_004089252.1 NZ_CP090547:c1060760-1060464 [Xylella fastidiosa subsp. sandyi]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T231170 NZ_CP126100:c2252787-2252536 [Bacillus halotolerans]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATTAAGGATTACTTCAGCAGCGAAGCACAAACACTAA
TCAATATAATATTCATAGTCACATGTATCATTGCCATATTGTTGTGGATCATCTATTTTGTATTCCTTAAACTAAGAAAT
AAGTCTCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT231170 WP_004089254.1 NZ_CP090547:c1060460-1060155 [Xylella fastidiosa subsp. sandyi]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT231170 NZ_CP126100:2252765-2252865 [Bacillus halotolerans]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: A0A060HBK9