Detailed information of TA system TA023117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 600548..601344
• Replicon: chromosome
• Accession: NC_011313
• Organism: Aliivibrio salmonicida LFI1238

Toxin (Protein)

• Gene name: -
• Uniprot ID: B6ERG1
• Locus tag: VSAL_RS18890
• Protein ID: WP_012551863.1
• Coordinates: 600814..601344 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VSAL_RS18885
• Protein ID: WP_044583569.1
• Coordinates: 600548..600817 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VSAL_RS18870	598055..598810	+	756	WP_012551859.1	membrane protein	-
VSAL_RS18875	599012..599725	+	714	WP_012551860.1	RES family NAD+ phosphorylase	-
VSAL_RS18880	599883..600317	+	435	WP_044583568.1	GNAT family N-acetyltransferase	-
VSAL_RS18885	600548..600817	+	270	WP_044583569.1	DUF1778 domain-containing protein	Antitoxin
VSAL_RS18890	600814..601344	+	531	WP_012551863.1	GNAT family N-acetyltransferase	Toxin
VSAL_RS18895	601353..602048	+	696	WP_012551864.1	hypothetical protein	-
VSAL_RS18900	602236..602490	+	255	WP_012551865.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VSAL_RS18905	602481..602795	+	315	WP_012551866.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VSAL_RS18910	603039..603389	+	351	WP_012551867.1	cupin domain-containing protein	-
VSAL_RS18915	603629..604099	+	471	WP_012551868.1	hypothetical protein	-
VSAL_RS18920	604092..604694	+	603	WP_012551869.1	putative adenosine monophosphate-protein transferase Fic	-
VSAL_RS18925	605386..605655	-	270	WP_012551870.1	type II toxin-antitoxin system YafQ family toxin	-
VSAL_RS18930	605648..605926	-	279	WP_012551871.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	595280..595999	719
	-	inside	Integron	-	-	593499..615361	21862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 19871.11 Da
• Isoelectric Point: 8.7689

>T23117 WP_012551863.1 NC_011313:600814-601344 [Aliivibrio salmonicida LFI1238]
VSYSKTFKELDKSQHDRASFDCGEKELNDFIQTQAAKHMQAGISRTMVLPASVPLPNQKYPICSFYSIAPSSISRDTLPQ
AMAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIKALEYLWEINSHMRAYAIVVDCLTEQAESFYAKYGFEVLCEING
RVRMFIPMKTVGQLFT

Nucleotide

• Length: 531 bp

>T23117 NC_011313:600814-601344 [Aliivibrio salmonicida LFI1238]
GTGAGTTATTCCAAGACTTTTAAAGAATTGGATAAATCACAACACGATAGAGCATCATTTGACTGCGGTGAAAAAGAGTT
AAATGATTTTATCCAAACTCAAGCCGCTAAACATATGCAAGCAGGTATCAGCCGCACTATGGTTTTGCCTGCTTCTGTGC
CTCTACCAAATCAAAAATATCCAATTTGTTCATTTTATAGTATTGCGCCAAGTTCAATTAGCCGTGATACGTTGCCACAA
GCCATGGCTAAAAAATTACCACGCTATCCAATTCCTGTTTTTCTTTTAGCTCAATTGGCAGTTCATAAAGAATTTCATGG
AAGTGGGTTAGGTAAAGTTAGCTTAATCAAAGCTCTAGAATATCTTTGGGAAATTAACTCTCACATGAGAGCATATGCCA
TTGTTGTTGATTGTTTAACTGAACAAGCTGAGTCATTCTACGCAAAATATGGTTTCGAGGTTCTTTGTGAAATCAATGGT
CGTGTAAGAATGTTCATTCCGATGAAAACAGTCGGTCAGTTATTCACTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9761.17 Da
• Isoelectric Point: 4.3894

>AT23117 WP_044583569.1 NC_011313:600548-600817 [Aliivibrio salmonicida LFI1238]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDSTQVISEYESITVEANVFDQFMIACDEAKAPNKALLGAA
AFTKSGEFK

Nucleotide

• Length: 270 bp

>AT23117 NC_011313:600548-600817 [Aliivibrio salmonicida LFI1238]
ATGGCTACTGCAAGACTTGATATCCGTTTGGATGAAGAAATCAAAGCTAAGGCTGAGAAAGCTTCAGCTTTACTTGGTTT
AAAAAGCTTAACTGAATACGTTGTTCGTTTAATGGACGAAGATTCAACTCAGGTGATTTCTGAATATGAAAGCATCACTG
TTGAAGCAAATGTATTTGACCAATTCATGATTGCTTGTGACGAGGCTAAAGCTCCGAATAAAGCATTACTTGGAGCTGCT
GCATTTACTAAAAGTGGTGAGTTTAAGTGA

Structures

Toxin

• AlphaFold DB: B6ERG1