Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5563338..5563963 Replicon chromosome
Accession NC_011283
Organism Klebsiella pneumoniae 342

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KPK_RS26105 Protein ID WP_008807903.1
Coordinates 5563338..5563721 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KPK_RS26110 Protein ID WP_004150355.1
Coordinates 5563721..5563963 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPK_RS26090 5560704..5561606 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KPK_RS26095 5561603..5562238 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KPK_RS26100 5562235..5563164 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KPK_RS26105 5563338..5563721 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KPK_RS26110 5563721..5563963 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
KPK_RS26115 5564168..5565085 + 918 WP_012543286.1 alpha/beta hydrolase -
KPK_RS26120 5565100..5566041 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KPK_RS26125 5566086..5566523 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
KPK_RS26130 5566520..5567380 - 861 WP_008807907.1 virulence factor BrkB family protein -
KPK_RS26135 5567374..5567973 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T23110 WP_008807903.1 NC_011283:c5563721-5563338 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T23110 NC_011283:c5563721-5563338 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT23110 WP_004150355.1 NC_011283:c5563963-5563721 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT23110 NC_011283:c5563963-5563721 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References