Detailed information of TA system TA231086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4133984..4134560
• Replicon: chromosome
• Accession: NZ_CP090535
• Organism: Salmonella enterica strain 2016089-SE

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: L0F58_RS20250
• Protein ID: WP_001131963.1
• Coordinates: 4134273..4134560 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: L0F58_RS20245
• Protein ID: WP_000063142.1
• Coordinates: 4133984..4134286 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F58_RS20230 (4130494)	4130494..4132644	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
L0F58_RS20235 (4132739)	4132739..4132942	+	204	WP_000460616.1	YbdD/YjiX family protein	-
L0F58_RS20240 (4132953)	4132953..4133909	+	957	WP_000187839.1	GTPase	-
L0F58_RS20245 (4133984)	4133984..4134286	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
L0F58_RS20250 (4134273)	4134273..4134560	-	288	WP_001131963.1	BrnT family toxin	Toxin
L0F58_RS20255 (4134829)	4134829..4135743	-	915	WP_000290512.1	restriction endonuclease	-
L0F58_RS20260 (4135941)	4135941..4139450	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T231086 WP_001131963.1 NZ_CP090535:c4134560-4134273 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T231086 NZ_CP125957:2200577-2200852 [Pseudomonas sp. G2-4]
ATGGAGTTGAAGTGGACGAGCAGGGCGCTCTCTGATATATCGCGGCTTTACGAATTTCTGGCGGCAGTGAATCAGCCTGC
GGCGGCACGAACGGTGCAGCAGCTCACCGCTGCACCGATCACGTTGCTGACCAACCCGCGTATCGGTGAACGCCTTGAAG
AGTTCGAGCCGCGGGGTGTGCGCCGAATTCAGATCGGCCACTATGAGATGCGTTACGAAATAATAGATTCCACCGTTTAC
CTGCTACGCCTGTGGCATACCCGCGAGAATCGATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT231086 WP_000063142.1 NZ_CP090535:c4134286-4133984 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT231086 NZ_CP125957:2200305-2200577 [Pseudomonas sp. G2-4]
ATGGCTACCACCACGAAAACCCGATCTGTCACGGCTCACGTCCCTGAGCAACTGGCCCAAAAAGTCGATTTGATGGCGGA
GCGTCTGGAACGCTCCAAAAACTGGATCGTTAAACAGGCACTGTCGGCTTGGATAGATCAGGAAGAAGAGCGTAGTCGCA
TGACTCATGAGGCCCTGGCTGATGTTGACGCTGGTCGGGTGATTGACCAGCAAGCTGTCCAAGCCTGGGCTGATAGCCTT
AGTACCGACACTCCGTTGCCGGTGCCGCGCTGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01