Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4133984..4134560 Replicon chromosome
Accession NZ_CP090535
Organism Salmonella enterica strain 2016089-SE

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag L0F58_RS20250 Protein ID WP_001131963.1
Coordinates 4134273..4134560 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag L0F58_RS20245 Protein ID WP_000063142.1
Coordinates 4133984..4134286 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0F58_RS20230 (4130494) 4130494..4132644 + 2151 WP_000379927.1 pyruvate/proton symporter BtsT -
L0F58_RS20235 (4132739) 4132739..4132942 + 204 WP_000460616.1 YbdD/YjiX family protein -
L0F58_RS20240 (4132953) 4132953..4133909 + 957 WP_000187839.1 GTPase -
L0F58_RS20245 (4133984) 4133984..4134286 - 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
L0F58_RS20250 (4134273) 4134273..4134560 - 288 WP_001131963.1 BrnT family toxin Toxin
L0F58_RS20255 (4134829) 4134829..4135743 - 915 WP_000290512.1 restriction endonuclease -
L0F58_RS20260 (4135941) 4135941..4139450 + 3510 WP_001043484.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T231086 WP_001131963.1 NZ_CP090535:c4134560-4134273 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T231086 NZ_CP125957:2200577-2200852 [Pseudomonas sp. G2-4]
ATGGAGTTGAAGTGGACGAGCAGGGCGCTCTCTGATATATCGCGGCTTTACGAATTTCTGGCGGCAGTGAATCAGCCTGC
GGCGGCACGAACGGTGCAGCAGCTCACCGCTGCACCGATCACGTTGCTGACCAACCCGCGTATCGGTGAACGCCTTGAAG
AGTTCGAGCCGCGGGGTGTGCGCCGAATTCAGATCGGCCACTATGAGATGCGTTACGAAATAATAGATTCCACCGTTTAC
CTGCTACGCCTGTGGCATACCCGCGAGAATCGATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT231086 WP_000063142.1 NZ_CP090535:c4134286-4133984 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT231086 NZ_CP125957:2200305-2200577 [Pseudomonas sp. G2-4]
ATGGCTACCACCACGAAAACCCGATCTGTCACGGCTCACGTCCCTGAGCAACTGGCCCAAAAAGTCGATTTGATGGCGGA
GCGTCTGGAACGCTCCAAAAACTGGATCGTTAAACAGGCACTGTCGGCTTGGATAGATCAGGAAGAAGAGCGTAGTCGCA
TGACTCATGAGGCCCTGGCTGATGTTGACGCTGGTCGGGTGATTGACCAGCAAGCTGTCCAAGCCTGGGCTGATAGCCTT
AGTACCGACACTCCGTTGCCGGTGCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References