Detailed information of TA system TA231066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid p2017005-292
• Accession: NZ_CP090534
• Organism: Salmonella enterica strain 2017005-SE

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: L0F61_RS25145
• Protein ID: WP_140780865.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: L0F61_RS25140
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F61_RS25115 (L0F61_25115)	79955..81196	+	1242	WP_140780866.1	VWA domain-containing protein	-
L0F61_RS25120 (L0F61_25120)	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
L0F61_RS25125 (L0F61_25125)	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
L0F61_RS25130 (L0F61_25130)	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
L0F61_RS25135 (L0F61_25135)	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
L0F61_RS25140 (L0F61_25140)	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
L0F61_RS25145 (L0F61_25145)	84381..85706	+	1326	WP_140780865.1	type II toxin-antitoxin system HipA family toxin	Toxin
L0F61_RS25150 (L0F61_25150)	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
L0F61_RS25155 (L0F61_25155)	86075..86458	+	384	WP_140780864.1	hypothetical protein	-
L0F61_RS25160 (L0F61_25160)	86498..87313	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
L0F61_RS25165 (L0F61_25165)	87567..87992	+	426	WP_001043843.1	hypothetical protein	-
L0F61_RS25170 (L0F61_25170)	88446..88682	+	237	Protein_85	type I restriction-modification system subunit M N-terminal domain-containing protein	-
L0F61_RS25175 (L0F61_25175)	88737..89036	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / oqxB / oqxA / blaCTX-M-14 / mcr-1.1 / qnrS2 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR	-	1..292358	292358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50291.28 Da
• Isoelectric Point: 6.1927

>T231066 WP_140780865.1 NZ_CP090534:84381-85706 [Salmonella enterica]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHVEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T231066 NZ_CP125903:c2253680-2253471 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT231066 WP_024136327.1 NZ_CP090534:84083-84376 [Salmonella enterica]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT231066 NZ_CP125903:c2253985-2253692 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1