Detailed information of TA system TA023106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4916125..4916701
• Replicon: chromosome
• Accession: NC_011283
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: KPK_RS23095
• Protein ID: WP_012542979.1
• Coordinates: 4916414..4916701 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: KPK_RS23090
• Protein ID: WP_012542978.1
• Coordinates: 4916125..4916427 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS23070	4911533..4912597	-	1065	WP_008807596.1	DUF2955 domain-containing protein	-
KPK_RS23075	4912587..4913654	-	1068	WP_012542976.1	HlyD family secretion protein	-
KPK_RS23080	4913661..4914125	-	465	WP_012542977.1	MarR family transcriptional regulator	-
KPK_RS28110	4914291..4914455	-	165	WP_008807599.1	DUF1127 domain-containing protein	-
KPK_RS23085	4914633..4916045	+	1413	WP_008807600.1	PLP-dependent aminotransferase family protein	-
KPK_RS23090	4916125..4916427	-	303	WP_012542978.1	BrnA antitoxin family protein	Antitoxin
KPK_RS23095	4916414..4916701	-	288	WP_012542979.1	BrnT family toxin	Toxin
KPK_RS23100	4916868..4917287	-	420	WP_008807601.1	FosA family fosfomycin resistance glutathione transferase	-
KPK_RS23105	4917281..4918189	-	909	WP_012542980.1	LysR family transcriptional regulator	-
KPK_RS23110	4918276..4919058	+	783	WP_012542981.1	NAD(P)H-dependent oxidoreductase	-
KPK_RS23115	4919200..4919784	+	585	WP_012542982.1	TetR/AcrR family transcriptional regulator	-
KPK_RS23120	4919806..4920609	+	804	WP_012542983.1	winged helix-turn-helix domain-containing protein	-
KPK_RS23125	4920606..4921124	+	519	WP_004182811.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11245.77 Da
• Isoelectric Point: 8.6574

>T23106 WP_012542979.1 NC_011283:c4916701-4916414 [Klebsiella variicola]
MPMEFEWDANKALSNLRKHGVRFEEAVLVFDDPRHLSRQERFENGEYRWQTIGLVHGILVILVAHSVRFESGTEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T23106 NC_011283:c4916701-4916414 [Klebsiella variicola]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCATTAAGTAATCTACGAAAACATGGAGTCCGGTTTGAAGAAGCGGT
ATTAGTCTTTGACGATCCGCGGCACCTTTCCCGCCAGGAGCGGTTTGAGAATGGTGAATATCGCTGGCAGACCATCGGTC
TTGTGCATGGGATCCTTGTTATCCTGGTGGCGCATTCGGTCCGTTTTGAAAGTGGAACTGAGGTTATTCGTATTATCAGC
GCCCGGAAGGCGGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11379.02 Da
• Isoelectric Point: 10.1284

>AT23106 WP_012542978.1 NC_011283:c4916427-4916125 [Klebsiella variicola]
MSMVKHKRDNTAALTAKRDAELKALASKADEDIDYSDIPATDDAQWSDAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT23106 NC_011283:c4916427-4916125 [Klebsiella variicola]
ATGAGCATGGTTAAACATAAACGCGACAATACGGCGGCGCTTACCGCTAAGCGTGATGCCGAACTCAAAGCGCTAGCGAG
CAAAGCCGATGAGGATATCGATTACAGCGATATCCCCGCTACAGACGATGCGCAATGGTCAGATGCCGTTCGCGGCAAAT
TTTTTCGACCGCTAAAAACACAGGCTTCTGTGCGCATTGATGCCGATGTCATGGAGTGGCTCAAACGTCCAGGAAAAGGC
TATCAAACTCGACTGAATGCGATTTTAAGAGAAGCGATGATTCGCGATCTTAATAAGAAATAG