Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4277975..4278594 Replicon chromosome
Accession NC_011283
Organism Klebsiella pneumoniae 342

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPK_RS20105 Protein ID WP_002892050.1
Coordinates 4278376..4278594 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KPK_RS20100 Protein ID WP_008805436.1
Coordinates 4277975..4278349 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPK_RS20090 4273130..4274323 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPK_RS20095 4274346..4277492 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
KPK_RS20100 4277975..4278349 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KPK_RS20105 4278376..4278594 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPK_RS20110 4278753..4279319 + 567 WP_008805435.1 maltose O-acetyltransferase -
KPK_RS31325 4279291..4279419 - 129 Protein_4083 hypothetical protein -
KPK_RS20115 4279456..4279926 + 471 WP_008805434.1 YlaC family protein -
KPK_RS20120 4279895..4281352 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
KPK_RS20125 4281453..4282151 + 699 WP_012542668.1 GNAT family N-acetyltransferase -
KPK_RS20130 4282148..4282288 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPK_RS20135 4282288..4282551 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T23105 WP_002892050.1 NC_011283:4278376-4278594 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T23105 NC_011283:4278376-4278594 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT23105 WP_008805436.1 NC_011283:4277975-4278349 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT23105 NC_011283:4277975-4278349 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References