Detailed information of TA system TA231032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4155231..4155807
• Replicon: chromosome
• Accession: NZ_CP090529
• Organism: Salmonella enterica strain 2008079-SE

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: L0F60_RS20380
• Protein ID: WP_001131963.1
• Coordinates: 4155520..4155807 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: L0F60_RS20375
• Protein ID: WP_000063142.1
• Coordinates: 4155231..4155533 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F60_RS20360 (4151741)	4151741..4153891	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
L0F60_RS20365 (4153986)	4153986..4154189	+	204	WP_000460616.1	YbdD/YjiX family protein	-
L0F60_RS20370 (4154200)	4154200..4155156	+	957	WP_000187839.1	GTPase	-
L0F60_RS20375 (4155231)	4155231..4155533	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
L0F60_RS20380 (4155520)	4155520..4155807	-	288	WP_001131963.1	BrnT family toxin	Toxin
L0F60_RS20385 (4156076)	4156076..4156990	-	915	WP_000290512.1	restriction endonuclease	-
L0F60_RS20390 (4157188)	4157188..4160697	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T231032 WP_001131963.1 NZ_CP090529:c4155807-4155520 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T231032 NZ_CP125897:c1920424-1920272 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT231032 WP_000063142.1 NZ_CP090529:c4155533-4155231 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT231032 NZ_CP125897:c1921048-1920449 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01