Detailed information of TA system TA231012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1215924..1216537
• Replicon: chromosome
• Accession: NZ_CP090527
• Organism: Cupriavidus metallidurans strain ZM16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q1LPG3
• Locus tag: LZQ98_RS17370
• Protein ID: WP_011515865.1
• Coordinates: 1216358..1216537 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LZQ98_RS17365
• Protein ID: WP_029310017.1
• Coordinates: 1215924..1216328 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZQ98_RS17350	1211314..1211847	+	534	WP_029308195.1	peptidylprolyl isomerase	-
LZQ98_RS17355	1211942..1212994	-	1053	WP_011515862.1	hypothetical protein	-
LZQ98_RS17360	1212991..1215741	-	2751	WP_224445916.1	DNA mismatch repair protein MutS	-
LZQ98_RS17365	1215924..1216328	-	405	WP_029310017.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LZQ98_RS17370	1216358..1216537	-	180	WP_011515865.1	type II toxin-antitoxin system HicA family toxin	Toxin
LZQ98_RS17375	1216741..1217562	-	822	WP_224445917.1	inositol monophosphatase family protein	-
LZQ98_RS17380	1217814..1218659	+	846	WP_224446129.1	RNA methyltransferase	-
LZQ98_RS17385	1218988..1219731	+	744	WP_029308190.1	serine O-acetyltransferase	-
LZQ98_RS17390	1219780..1220571	-	792	WP_224445918.1	UDP-2,3-diacylglucosamine diphosphatase	-
LZQ98_RS17395	1220640..1221140	-	501	WP_011515870.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6790.07 Da
• Isoelectric Point: 12.3707

>T231012 WP_011515865.1 NZ_CP090527:c1216537-1216358 [Cupriavidus metallidurans]
MHSSTLIRLLEQKGWKLVRTHGSHFTFKHPRHMLIVTVPHPKKDLPTGTVRNILRTAGL

Nucleotide

• Length: 180 bp

>T231012 NZ_CP125857:827617-827871 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGCTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGTCGTGATTCTGAAGATGCTGTGGTTATGTCCTTAGATTACTACAATAGCCTTATGGAGACAGTGCACT
TACTACGCTCTCCTCAAAACGCTGAACACTTAAACCGTTCTATAGCACAGTACCGTGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14618.79 Da
• Isoelectric Point: 4.9285

>AT231012 WP_029310017.1 NZ_CP090527:c1216328-1215924 [Cupriavidus metallidurans]
MDFPIAIHKDANSVYGVTVPDVAGCYSAGESIEEAVRNAREAITTHVVALLSLGEEVVLKTTPIGTLMAQPEYADAIWAL
ADIDMSKLDARPERINISLPRFVLHKIDMFVERRHETRSGFLARVALDAIAGSV

Nucleotide

• Length: 405 bp

>AT231012 NZ_CP125857:827864-828133 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAGCGTTTACTGTCGTGGACTGATGACGCTTGGGACGACTACCTGTATTGGCAAACTCAAGACAAAAA
AACACTCAAGCGTATCAATAAACTTATCAATGATGTTAAACGTTCTCCATTTGAGGGTATCGGTAAACCTGAGCCATTGA
AAGAGAACTTATCTGGTTTTTGGTCCCGTCGTGTTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAGGTCATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Structures

Toxin

• AlphaFold DB: A0A2L0WZI7