Detailed information of TA system TA231010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 6536..7125
• Replicon: plasmid pXAG39.ICMP5732
• Accession: NZ_CP090524
• Organism: Xanthomonas citri pv. glycines strain ICMP5732

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q32X98
• Locus tag: LMJ37_RS23295
• Protein ID: WP_012477455.1
• Coordinates: 6793..7125 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: Q32X99
• Locus tag: LMJ37_RS23290
• Protein ID: WP_012477454.1
• Coordinates: 6536..6793 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMJ37_RS23250 (LMJ37_23250)	2259..3179	-	921	WP_012477431.1	replication protein RepA	-
LMJ37_RS23255 (LMJ37_23255)	3245..3670	-	426	WP_234746980.1	serine protease	-
LMJ37_RS23260 (LMJ37_23260)	3564..4052	-	489	WP_234746984.1	serine protease	-
LMJ37_RS23265 (LMJ37_23265)	4049..4207	-	159	WP_154666355.1	hypothetical protein	-
LMJ37_RS23270 (LMJ37_23270)	4430..4684	-	255	WP_012477434.1	hypothetical protein	-
LMJ37_RS23275 (LMJ37_23275)	4684..5334	-	651	WP_012477435.1	ParA family protein	-
LMJ37_RS23280 (LMJ37_23280)	5401..6046	-	646	Protein_8	recombinase family protein	-
LMJ37_RS23285 (LMJ37_23285)	6043..6270	-	228	WP_012477437.1	hypothetical protein	-
LMJ37_RS23290 (LMJ37_23290)	6536..6793	+	258	WP_012477454.1	plasmid stable inheritance protein I	Antitoxin
LMJ37_RS23295 (LMJ37_23295)	6793..7125	+	333	WP_012477455.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LMJ37_RS23300 (LMJ37_23300)	7214..8032	-	819	WP_012477456.1	hypothetical protein	-
LMJ37_RS23305 (LMJ37_23305)	8060..8647	-	588	WP_234746982.1	hypothetical protein	-
LMJ37_RS23310 (LMJ37_23310)	9072..10235	-	1164	WP_234746976.1	relaxase/mobilization nuclease domain-containing protein	-
LMJ37_RS23315 (LMJ37_23315)	10237..10539	-	303	WP_012477458.1	hypothetical protein	-
LMJ37_RS23320 (LMJ37_23320)	10827..11186	+	360	WP_234746978.1	hypothetical protein	-
LMJ37_RS23325 (LMJ37_23325)	11259..11939	+	681	WP_012477460.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38602	38602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11581.24 Da
• Isoelectric Point: 9.1684

>T231010 WP_012477455.1 NZ_CP090524:6793-7125 [Xanthomonas citri pv. glycines]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Nucleotide

• Length: 333 bp

>T231010 NZ_CP125856:c1056711-1056412 [Vibrio parahaemolyticus]
ATGCAAAAGAACAAATACAAGCTAAGTAACTTAGCGCAATCTCACTTGCGCAAAGTAAAAGACTACACTGTTGAAAACTT
TTCTGAATTACAGTGGCGTAACTACAAAGATACTCTGCTATCAGGGTTTCAGATGCTAGCTGATAATCCAGACTTAGGAA
GAAGTTGTGACGAGATATACCCAAATGGCTTTTTTTTCCCGATTGGAAAACACACAGCTTATTTCATCAAAGAAGATGAC
TTTATCTTAGTCGTTGGTGTCTTAGGTCAATCACAGCTACCCCAGAATCATCTGAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8951.34 Da
• Isoelectric Point: 5.5491

>AT231010 WP_012477454.1 NZ_CP090524:6536-6793 [Xanthomonas citri pv. glycines]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Nucleotide

• Length: 258 bp

>AT231010 NZ_CP125856:c1056961-1056719 [Vibrio parahaemolyticus]
ATGCATACATTAACAGCCAATGATGCCAAACGAAATTTCGGCGAACTACTTCTGAGCGCACAACGCGAGCCAGTAAAAAT
CAGCAAAAACAGTAAAGATACTGTCGTTGTCATGTCCATTCGTGACTTTGAAGAGCTAGAAGCTATGAAAGTTGAGTACC
TCAAACACTGCTTTAAGTCAGCTAAAGAAGACTTGAACAATGGAAACACTGTTGATGGTGAAAGCTTTCTGAACTCGCTG
TAA

Structures

Toxin

• AlphaFold DB: A0A7Z6Z9Y5

Antitoxin

• AlphaFold DB: A0A1D9EPT3