Detailed information of TA system TA230976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1025945..1026699
• Replicon: chromosome
• Accession: NZ_CP090513
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak 8

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LZ751_RS04415
• Protein ID: WP_021358673.1
• Coordinates: 1025945..1026424 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: LZ751_RS04420
• Protein ID: WP_004085396.1
• Coordinates: 1026427..1026699 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ751_RS04405 (LZ751_04405)	1022798..1023604	+	807	WP_012337796.1	oxidoreductase	-
LZ751_RS04410 (LZ751_04410)	1023744..1025609	+	1866	WP_012337797.1	DUF5710 domain-containing protein	-
LZ751_RS04415 (LZ751_04415)	1025945..1026424	-	480	WP_021358673.1	GNAT family N-acetyltransferase	Toxin
LZ751_RS04420 (LZ751_04420)	1026427..1026699	-	273	WP_004085396.1	DUF1778 domain-containing protein	Antitoxin
LZ751_RS04425 (LZ751_04425)	1027051..1027956	-	906	WP_004085395.1	AEC family transporter	-
LZ751_RS04430 (LZ751_04430)	1028072..1028690	+	619	Protein_864	IS607 family transposase	-
LZ751_RS04435 (LZ751_04435)	1028918..1030951	+	2034	WP_228762228.1	glycoside hydrolase family 3 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (33-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17645.42 Da
• Isoelectric Point: 9.9283

>T230976 WP_021358673.1 NZ_CP090513:c1026424-1025945 [Xylella fastidiosa subsp. multiplex]
MQISTPHSLTAAHRLDEFNCGEPSLDDWLKRRALTNHLNGASRTFVVIDANQYVLGYYALAAGAVAHQEATRAIRRNMPD
PVPVMVLGRLAVDTRAQGIKLGAALLKDTVTRVQSIAENAGVRALVVHALNERAKQFYEYYGFRASPMHLMTLMLPIKF

Nucleotide

• Length: 480 bp

>T230976 NZ_CP125762:c1620195-1620016 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10264.77 Da
• Isoelectric Point: 5.0488

>AT230976 WP_004085396.1 NZ_CP090513:c1026699-1026427 [Xylella fastidiosa subsp. multiplex]
MRDASINLRAMPEQRDLIDQAANLLGKNRSDFMLEAACERAKAIILDQVFFNLDEERFRQFTVLLNAPQDTNPGLNRLMA
IKAPWSISNT

Nucleotide

• Length: 273 bp

>AT230976 NZ_CP125762:1619869-1620092 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAAACTACCCTTTAATA
GGAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC