Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 472302..472888 Replicon chromosome
Accession NZ_CP090513
Organism Xylella fastidiosa subsp. multiplex strain Red Oak 8

Toxin (Protein)


Gene name hicA Uniprot ID Q87BV5
Locus tag LZ751_RS01975 Protein ID WP_004089517.1
Coordinates 472634..472888 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B2I6C9
Locus tag LZ751_RS01970 Protein ID WP_004085025.1
Coordinates 472302..472637 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ751_RS01940 (LZ751_01940) 469076..469609 - 534 WP_021358584.1 terminase small subunit -
LZ751_RS01945 (LZ751_01945) 469782..470006 - 225 WP_012337656.1 hypothetical protein -
LZ751_RS01950 (LZ751_01950) 470008..470469 - 462 WP_012337657.1 hypothetical protein -
LZ751_RS01955 (LZ751_01955) 470459..470788 - 330 WP_012337658.1 phage holin family protein -
LZ751_RS01960 (LZ751_01960) 470781..471281 - 501 WP_012337659.1 lysozyme -
LZ751_RS01965 (LZ751_01965) 471381..472112 - 732 WP_012337660.1 site-specific DNA-methyltransferase -
LZ751_RS01970 (LZ751_01970) 472302..472637 - 336 WP_004085025.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LZ751_RS01975 (LZ751_01975) 472634..472888 - 255 WP_004089517.1 type II toxin-antitoxin system HicA family toxin Toxin
LZ751_RS01980 (LZ751_01980) 472941..473663 - 723 WP_012337661.1 hypothetical protein -
LZ751_RS01985 (LZ751_01985) 473606..473953 - 348 WP_162010044.1 hypothetical protein -
LZ751_RS01990 (LZ751_01990) 474141..475409 + 1269 WP_012337662.1 DNA polymerase -
LZ751_RS01995 (LZ751_01995) 475406..475684 + 279 WP_012337663.1 VRR-NUC domain-containing protein -
LZ751_RS02000 (LZ751_02000) 475686..475991 - 306 WP_012337664.1 hypothetical protein -
LZ751_RS02005 (LZ751_02005) 476076..477494 + 1419 WP_012337665.1 DEAD/DEAH box helicase -
LZ751_RS02010 (LZ751_02010) 477491..477736 + 246 WP_012337666.1 DUF4224 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 446882..481254 34372


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-80)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9639.14 Da        Isoelectric Point: 11.2766

>T230975 WP_004089517.1 NZ_CP090513:c472888-472634 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Download         Length: 255 bp

>T230975 NZ_CP125762:c1619605-1619507 [Bacillus subtilis]
ATGCTTATGCTTGCTTTCGGGTCATTTATAATTGCCCTGTTGACTTATATAAAAGAAGAAATAGACCCACCCCTTGAGCT
CGGCAAAGTAAAAGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12324.07 Da        Isoelectric Point: 5.6556

>AT230975 WP_004085025.1 NZ_CP090513:c472637-472302 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Download         Length: 336 bp

>AT230975 NZ_CP125762:1619351-1619554 [Bacillus subtilis]
TTAGAAGTAATTCAAACCGTACCAAATGGAAACATGTATTTCTTTCAAAAGAAATGCATAAAATAAAAGAGACCCGGTTG
CCGCCGGGTCAGTATAAATGTTGGCCCATCAAGAGGGCTTGGCTTAACAACTTCATGAAAGTATTTAGGATAGACTTTAC
CCTTTTACTTTGCCGAGCTCAAGGGGTGGGTCTATTTCTTCTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q87BV5

Antitoxin

Source ID Structure
AlphaFold DB A0A060HAK5

References