Detailed information of TA system TA230971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1290..1876
• Replicon: plasmid pXF26-Oak35874
• Accession: NZ_CP090512
• Organism: Xylella fastidiosa subsp. multiplex strain Oak 35874

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LZ754_RS12105
• Protein ID: WP_038211573.1
• Coordinates: 1550..1876 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LZ754_RS12100
• Protein ID: WP_038211571.1
• Coordinates: 1290..1553 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ754_RS12095 (LZ754_12095)	130..1230	+	1101	WP_128723750.1	zeta toxin family protein	-
LZ754_RS12100 (LZ754_12100)	1290..1553	+	264	WP_038211571.1	antitoxin	Antitoxin
LZ754_RS12105 (LZ754_12105)	1550..1876	+	327	WP_038211573.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LZ754_RS12110 (LZ754_12110)	1955..2917	-	963	WP_234500013.1	relaxase/mobilization nuclease domain-containing protein	-
LZ754_RS12115 (LZ754_12115)	3131..3418	-	288	WP_020852266.1	DUF4190 domain-containing protein	-
LZ754_RS12120 (LZ754_12120)	3466..4572	-	1107	WP_038211579.1	hypothetical protein	-
LZ754_RS12125 (LZ754_12125)	4585..6861	-	2277	WP_020852264.1	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26361	26361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11714.61 Da
• Isoelectric Point: 8.5480

>T230971 WP_038211573.1 NZ_CP090512:1550-1876 [Xylella fastidiosa subsp. multiplex]
MKRGDVYMVDLEPTAGHEQRGHRPVVVISSERFNRLTSCPVILPITNGGEFASRLGFAVELLGTMTTGIVRCDQPRALDL
LARNARKVESLPPGVLADVLAKAGTIFQ

Nucleotide

• Length: 327 bp

>T230971 NZ_CP125736:73410-73715 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9377.83 Da
• Isoelectric Point: 4.3588

>AT230971 WP_038211571.1 NZ_CP090512:1290-1553 [Xylella fastidiosa subsp. multiplex]
MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGAAVGLVITDGRLVIEPVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAP
AVGRELI

Nucleotide

• Length: 264 bp

>AT230971 NZ_CP125736:73190-73408 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GATAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA